Duchatel J P, Maghuin-Rogister G
Ann Rech Vet. 1985;16(1):93-7.
Anti-zeranol 7-hemisuccinate-bovine serum albumin was raised in rabbits. This antiserum was used in a radio-immunoassay of zeranol residues in urine and plasma of calves implanted with Forplix. The antibody was specific for zeranol but cross-reacted with its metabolite zearalenone and the mycotoxin zearalenone. Detection limits in plasma and in urine were 100 pg/ml and 1 ng/ml respectively. In veal calves treated with zeranol containing implant, no residues were detected in plasma even if plasma proteins were hydrolysed with pronase before the radio-immunoassay. Free and conjugated residues in urine were easily measured. The urine concentration of conjugated residues increased markedly after the 20th day of treatment and was still high (19 ppb) at day 40.
抗玉米赤霉醇7-半琥珀酸酯-牛血清白蛋白在兔体内产生。该抗血清用于对植入Forplix的犊牛尿液和血浆中玉米赤霉醇残留进行放射免疫测定。该抗体对玉米赤霉醇具有特异性，但与它的代谢物玉米赤霉烯酮和霉菌毒素玉米赤霉烯酮发生交叉反应。血浆和尿液中的检测限分别为100 pg/ml和1 ng/ml。在用含玉米赤霉醇的植入物处理的犊牛中，即使在放射免疫测定前用链霉蛋白酶水解血浆蛋白，血浆中也未检测到残留。尿液中的游离和结合残留易于测定。处理第20天后，结合残留的尿液浓度显著增加，在第40天时仍很高（19 ppb）。
