类胡萝卜素对蓝光诱导的人视网膜色素上皮细胞损伤的保护作用。
Protective effects of carotenoids against blue light induced-cellular damage in human retinal pigment epithelium.
作者信息
Won Jong Hoon, Sitnikov Dmitri, Hong Jina
机构信息
Amway Corporation, Amway I&S, 7575 Fulton St E, Ada, MI 49355 USA.
Nutrilite Health Institute, Amway I&S, 5600 Beach Blvd, Buena Park, CA 90621 USA.
出版信息
Food Sci Biotechnol. 2025 Jan 27;34(8):1713-1723. doi: 10.1007/s10068-024-01757-z. eCollection 2025 Apr.
UNLABELLED
The retinal pigmented epithelium (RPE) is constantly exposed to visible light, including blue light (BL) that creates reactive oxygen species (ROS), which are harmful to DNA and induce cellular senescence. Carotenoids are recognized for their antioxidant properties, but their protective effect on DNA repair and cellular senescence under BL induced oxidative stress has not been evaluated. After BL irradiation, the positive senescence-associated-β-galactosidase (SA-β-gal) staining, and gene expression of and were upregulated in ARPE-19 cells. Pretreatment with carotenoids reduced ROS, p-H2A.X nuclear foci, and SA-β-gal positive cells induced by BL irradiation. Furthermore, pretreatment with carotenoids reduced the secretion of IL-6 and VEGF triggered by BL. Since increased senescent cells and secretion of IL-6 and VEGF are involved in age-related macular degeneration pathogenesis, our results support that carotenoid supplementation has a potential role in protecting the eyes from the deleterious effects of excessive BL exposure.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-024-01757-z.
未标注
视网膜色素上皮（RPE）持续暴露于可见光，包括会产生活性氧（ROS）的蓝光（BL），活性氧对DNA有害并诱导细胞衰老。类胡萝卜素因其抗氧化特性而闻名，但其在蓝光诱导的氧化应激下对DNA修复和细胞衰老的保护作用尚未得到评估。蓝光照射后，衰老相关β-半乳糖苷酶（SA-β-gal）阳性染色以及p16和p21的基因表达在ARPE-19细胞中上调。用类胡萝卜素预处理可减少蓝光照射诱导的活性氧、p-H2A.X核灶和SA-β-gal阳性细胞。此外，用类胡萝卜素预处理可减少蓝光触发的白细胞介素-6（IL-6）和血管内皮生长因子（VEGF）的分泌。由于衰老细胞增加以及IL-6和VEGF的分泌与年龄相关性黄斑变性的发病机制有关，我们的结果支持补充类胡萝卜素在保护眼睛免受过量蓝光暴露的有害影响方面具有潜在作用。
补充信息
在线版本包含可在10.1007/s10068-024-01757-z获取的补充材料。
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