Yang Weimin, Wang Qingkai, Zhang Bin, Leung Ross Ka-Kit, Deng Kai, Geng Shuangshuang, Xu Jinfeng, Qiao Yu, Gao Hui, Li Dongchuan, Cai Liyi
Hebei Maternity Hospital, Shijiazhuang City, Hebei Province, China.
Shi Jiazhuang Technology Innovation Center of Precision Prevention and Control of Birth Defects, Shijiazhuang City, Hebei Province, China.
J Assist Reprod Genet. 2025 Mar 28. doi: 10.1007/s10815-025-03450-4.
To examine the potential association between blastocyst hatching status and pregnancy outcomes following single blastocyst transfer.
This is a retrospective cohort. We screened all frozen-thawed single blastocyst transfer cycles from January 1, 2020, to April 30, 2022, at the authors' center. The hatching status was graded into four categories: unhatched, early hatching (hatched portion < the diameter of zona pellucida), late hatching (hatched portion > the diameter of zona pellucida), and fully hatched. Multivariate logistic regression was used to examine the association between hatching status and pregnancy outcomes (clinical pregnancy and live birth).
The final analysis included 906 cycles. The hatching status was unhatched in 116 cycles, early hatching in 556 cycles, late hatching in 197 cycles, and fully hatched in 37 cycles. The clinical pregnancy rate was 54.3%, 63.3%, 74.6%, and 54.1%, respectively (p = 0.001) in the unhatched, early-hatching, late-hatching, and fully hatched groups, respectively. The live birth rate was 39.7%, 51.6%, 58.3%, and 40.5%, respectively (p = 0.008). In pairwise comparisons, the late-hatching group had significantly higher rates of clinical pregnancy and live birth versus the unhatched category (p = 0.001 and p = 0.008, respectively). In multivariate logistic regression analysis, embryo hatching status, the duration until blastocyst formation, the grading of blastocyst cells, and the thickness of the endometrium were associated with clinical pregnancy and live birth.
After adjusting for confounding factors, late-hatching status of the blastocysts was associated with a higher rate of clinical pregnancy and live birth.
探讨单囊胚移植后囊胚孵化状态与妊娠结局之间的潜在关联。
这是一项回顾性队列研究。我们筛选了2020年1月1日至2022年4月30日在作者所在中心进行的所有冻融单囊胚移植周期。孵化状态分为四类：未孵化、早期孵化（孵化部分<透明带直径）、晚期孵化（孵化部分>透明带直径）和完全孵化。采用多因素逻辑回归分析来研究孵化状态与妊娠结局（临床妊娠和活产）之间的关联。
最终分析纳入906个周期。116个周期囊胚未孵化，556个周期为早期孵化，197个周期为晚期孵化，37个周期为完全孵化。未孵化、早期孵化、晚期孵化和完全孵化组的临床妊娠率分别为54.3%、63.3%、74.6%和54.1%（p = 0.001）。活产率分别为39.7%、51.6%、58.3%和40.5%（p = 0.008）。在两两比较中，晚期孵化组的临床妊娠率和活产率显著高于未孵化组（分别为p = 0.001和p = 0.008）。在多因素逻辑回归分析中，胚胎孵化状态、囊胚形成所需时间、囊胚细胞分级和子宫内膜厚度与临床妊娠和活产相关。
在调整混杂因素后，囊胚的晚期孵化状态与较高的临床妊娠率和活产率相关。
