Dai Lu, Wan Jiajia, Zhang Rui, Xie Tingting, Jia Yizhen, Lu Zhichao, Zhang Fuxian, Ke Wenting, Liu Feng, Lei Liancheng
College of Animal Science and Technology, Yangtze University, Jingzhou, Hubei, China.
College of Animal Science and Technology, Yangtze University, Jingzhou, Hubei, China.
Vet Microbiol. 2025 May;304:110491. doi: 10.1016/j.vetmic.2025.110491. Epub 2025 Mar 23.
Glaesserella parasuis (synonym Haemophilus parasuis) is the pathogenic agent of Glässer's disease and causes huge economic losses in the world's swine industry. Glaesserella parasuis (G. parasuis) can be divided into 15 serotypes, and the cross-protection effect of existing vaccines is not satisfactory. Therefore, the development of a vaccine to prevent multiple serotypes of G. parasuis infection is of great significance for the prevention and treatment of Glässer's disease, but still faces many difficulties. In this study, the B-cell, CTL and Th cell epitopes of CtdB, CtbC, OppA, TbpB, HxuC, D15, Omp2 and Omp5 proteins were predicted by bioinformatics method, and multi-epitope proteins Xlc and Ddc were obtained by concatenating epitopes through linkers. After immunization with Xlc and Ddc, the levels of antibodies, IL-4, and IFN-γ in mice were significantly increased. The protective rates of Xlc+Ddc immunized mice against G. parasuis serotypes 4, 5, and 10 were 62.5 %, 75 %, and 87.5 %, respectively, which were higher than those of Xlc (37.5 %, 62.5 %, and 87.5 %) and Ddc (75 %, 25 %, and 50 %). Overall, the combination of multi-epitope proteins Xlc and Ddc had good immunogenicity and strong cross-protection against G. parasuis serotypes 4, 5, and 10. These results indicated that multi-epitope proteins Xlc and Ddc can serve as candidate subunit vaccines against G. parasuis infection.
副猪嗜血杆菌（同义词：猪副嗜血杆菌）是格拉泽氏病的病原体，在全球养猪业中造成巨大经济损失。副猪嗜血杆菌（G. parasuis）可分为15个血清型，现有疫苗的交叉保护效果并不理想。因此，开发一种能预防多种血清型副猪嗜血杆菌感染的疫苗对格拉泽氏病的防治具有重要意义，但仍面临诸多困难。在本研究中，通过生物信息学方法预测了CtdB、CtbC、OppA、TbpB、HxuC、D15、Omp2和Omp5蛋白的B细胞、CTL和Th细胞表位，并通过连接子连接表位获得了多表位蛋白Xlc和Ddc。用Xlc和Ddc免疫小鼠后，小鼠体内抗体、IL-4和IFN-γ水平显著升高。Xlc+Ddc免疫的小鼠对副猪嗜血杆菌血清型4、5和10的保护率分别为62.5%、75%和87.5%，高于Xlc（37.5%、62.5%和87.5%）和Ddc（75%、25%和50%）。总体而言，多表位蛋白Xlc和Ddc的组合具有良好的免疫原性，对副猪嗜血杆菌血清型4、5和10具有较强的交叉保护作用。这些结果表明，多表位蛋白Xlc和Ddc可作为预防副猪嗜血杆菌感染的候选亚单位疫苗。
