Holzman Robert M, Hsieh Emmelyn, Dietrich Jaclyn, Cline Kirsten, Dale Jennifer L, Wagner Regina, Vagnone Paula Snippes, Kollmann Leslie, Cole Stephen D
Department of Clinical Sciences and Advanced Medicine, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA.
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA.
Am J Vet Res. 2025 Mar 28;86(6). doi: 10.2460/ajvr.25.01.0024. Print 2025 Jun 1.
To investigate the patterns of environmental contamination by carbapenemase-producing Enterobacterales (CPE) at veterinary hospitals in or around Philadelphia, PA, and to determine genetic relatedness between isolates.
Between September 2022 and August 2023, participating hospitals (n = 15) completed a survey regarding demographic data, antimicrobial stewardship efforts and prescribing practices, and infection prevention practices. Samples were collected from a variety of environmental surfaces (n = 311) at each hospital and then enriched and cultured on selective agar to screen for CPE. Confirmed CPE isolates were whole genome sequenced.
Of the 15 hospitals that participated in the study, 6 hospitals (40%) had CPE isolated from at least 1 site. All CPE harbored the blaNDM-7 carbapenemase gene. Of the 24 confirmed CPE isolates, 21 isolates (79%) belonged to a clonal cluster of Enterobacter hormaechei subsp xiangfangensis (single nucleotide polymorphism cluster PDS000141048 on National Center for Biotechnology Information Pathogen Detection). The PDS000141048 cluster was represented at 5 of the 6 hospitals from which CPE was isolated. Additionally, the PDS000141048 cluster included previously uploaded isolates from a biosecurity screening program at 1 of the hospitals in the study, as well as isolates from clinical specimens from outside of the Philadelphia area.
These data suggest that CPE can be common in the veterinary hospital environment. We also detected the presence of a network of transmission of carbapenemase-producing E hormaechei among veterinary hospitals in the greater Philadelphia region.
This finding highlights the importance of environmental infection prevention measures in companion animal veterinary medicine and the need for collaboration across veterinary facilities to prevent the spread of CPE.
调查宾夕法尼亚州费城及其周边地区兽医医院中产碳青霉烯酶肠杆菌科细菌（CPE）的环境污染模式，并确定分离株之间的遗传相关性。
在2022年9月至2023年8月期间，参与研究的医院（n = 15）完成了一项关于人口统计学数据、抗菌药物管理措施和处方习惯以及感染预防措施的调查。在每家医院从各种环境表面（n = 311）采集样本，然后在选择性琼脂上进行富集培养以筛选CPE。对确诊的CPE分离株进行全基因组测序。
在参与研究的15家医院中，有6家医院（40%）至少从1个部位分离出CPE。所有CPE均携带blaNDM - 7碳青霉烯酶基因。在24株确诊的CPE分离株中，21株（79%）属于阪崎肠杆菌xiangfangensis亚种的一个克隆簇（美国国立生物技术信息中心病原体检测上的单核苷酸多态性簇PDS000141048）。PDS000141048簇在分离出CPE的6家医院中的5家出现。此外，PDS000141048簇包括来自研究中1家医院生物安全筛查项目先前上传的分离株，以及来自费城地区以外临床标本的分离株。
这些数据表明CPE在兽医医院环境中可能很常见。我们还检测到在大费城地区的兽医医院中存在产碳青霉烯酶阪崎肠杆菌的传播网络。
这一发现凸显了伴侣动物兽医学中环境感染预防措施的重要性，以及兽医机构之间合作预防CPE传播的必要性。
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