Ding Jianing, Xu Chaoli, Du Yan, Liu Xiao, Chen Jingmin, Li Zhe, Long Jing, Sheng Yukun, Jin Wenjie, Xu Dan, Zhou Libin
Biophysics Group, Biomedical Center, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, 730000, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
BMC Genomics. 2025 Mar 28;26(1):308. doi: 10.1186/s12864-025-11488-y.
Ionizing radiation (IR) is an abiotic stress factor that can be not only a means to explore plant resistance but also a potent mutagen in agricultural breeding. The diverse physical parameters of different types of IR result in varying effects on plants, which in turn leads to differences in the spectrum of genetic variations in the offspring. Investigating plant response mechanisms to different IR is crucial for enhancing plant resistance and comprehending of the differences in mutation generation from various physical mutagenic sources in mutation breeding. Nevertheless, the mechanism underlying the complex responses of plants to different IR are not yet fully comprehended.
we conducted transcriptome sequencing on rice seedlings that exhibited a relative root length of approximately 69% after being exposed to carbon ion beams (CIBs) and X-ray respectively. The results revealed that X-ray induced a greater number of differentially expressed genes (DEGs) than CIBs, with 5681 and 2198 DEGs were identified respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that DNA replication, damage repair, phytohormone signaling, and antioxidant pathways were implicated in the response of rice seedling to IR. These pathways demonstrated diverse response patterns following different IR. Additionally, through two IR with different linear energy transfer (LET), we found some common DEGs that contribute to the radiation response in rice seedlings, such as LOC4331062, LOC4333870.
This study offers insights into the molecular transcriptomic mechanisms underlying the impacts of IR on rice seedlings. It provides a new perspective for further exploration of irradiation-induced damage repair factors and understanding the reasons for the differences in mutations created by different mutagenic sources in plants.
电离辐射（IR）是一种非生物胁迫因子，它不仅是探索植物抗性的手段，也是农业育种中一种强大的诱变剂。不同类型电离辐射的各种物理参数对植物产生不同的影响，进而导致后代遗传变异谱的差异。研究植物对不同电离辐射的响应机制对于增强植物抗性以及理解诱变育种中各种物理诱变源产生突变的差异至关重要。然而，植物对不同电离辐射复杂响应的潜在机制尚未完全理解。
我们对分别暴露于碳离子束（CIBs）和X射线后相对根长约为69%的水稻幼苗进行了转录组测序。结果显示，X射线诱导的差异表达基因（DEGs）数量比碳离子束更多，分别鉴定出5681个和2198个差异表达基因。基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析表明，DNA复制、损伤修复、植物激素信号传导和抗氧化途径与水稻幼苗对电离辐射的响应有关。这些途径在不同的电离辐射后表现出不同的响应模式。此外，通过两种具有不同线能量转移（LET）的电离辐射，我们发现了一些有助于水稻幼苗辐射响应的共同差异表达基因，如LOC4331062、LOC4333870。
本研究揭示了电离辐射对水稻幼苗影响的分子转录组机制。它为进一步探索辐射诱导的损伤修复因子以及理解植物中不同诱变源产生突变差异的原因提供了新的视角。
