豚鼠肝脏微粒体中细胞色素P-450依赖的尼古丁氧化作用。用抗苯巴比妥诱导型细胞色素P-450抗体的免疫化学证据。

Cytochrome P-450-dependent nicotine oxidation by liver microsomes of guinea pigs. Immunochemical evidence with antibody against phenobarbital-inducible cytochrome P-450.

作者信息

Nakayama H, Nakashima T, Kurogochi Y

出版信息

Biochem Pharmacol. 1985 Jul 1;34(13):2281-6. doi: 10.1016/0006-2952(85)90782-8.

Abstract

When guinea pigs were treated with phenobarbital (PB), the specific activity of liver microsomal nicotine oxidase increased by 42%. PB-inducible cytochrome P-450 (PB-P-450) was purified to homogeneity from liver microsomes of PB-treated guinea pigs. Purified PB-P-450 catalyzed nicotine oxidation when reconstituted with NADPH-P-450 reductase and phospholipid system. Antibody prepared against the purified PB-P-450 formed single precipitation lines with both purified PB-P-450 and microsomal components in livers of PB-treated guinea pigs, and both precipitation lines fused. The antibody against PB-P-450 strongly inhibited nicotine oxidation in the reconstituted system. The antibody also inhibited liver microsomal nicotine oxidase activities in PB-treated and untreated guinea pigs by about 30% and less than 5% respectively. About 45% of total P-450 in liver microsomes of PB-treated guinea pigs was precipitated by the antibody. These results show that PB-P-450 participates in liver microsomal nicotine oxidation in PB-treated guinea pigs but not in untreated control animals.

摘要

当豚鼠用苯巴比妥（PB）处理时，肝微粒体尼古丁氧化酶的比活性增加了42%。从经PB处理的豚鼠肝脏微粒体中纯化出PB诱导型细胞色素P-450（PB-P-450），使其达到同质。纯化的PB-P-450在与NADPH-P-450还原酶和磷脂系统重构时催化尼古丁氧化。针对纯化的PB-P-450制备的抗体与纯化的PB-P-450以及经PB处理的豚鼠肝脏中的微粒体成分形成单一沉淀线，且两条沉淀线融合。针对PB-P-450的抗体在重构系统中强烈抑制尼古丁氧化。该抗体还分别抑制经PB处理和未处理的豚鼠肝脏微粒体尼古丁氧化酶活性约30%和不到5%。经PB处理的豚鼠肝脏微粒体中约45%的总P-450被该抗体沉淀。这些结果表明，PB-P-450参与经PB处理的豚鼠肝脏微粒体尼古丁氧化，但不参与未处理的对照动物。