Das P K, Guha S R
Biochem Pharmacol. 1985 Aug 1;34(15):2663-7. doi: 10.1016/0006-2952(85)90564-7.
The metabolic significance of indole-3-acetaldehyde in the process of in vitro pigment formation from tryptamine in the presence of guinea-pig liver mitochondria was investigated. Among the four type selective MAO inhibitors used, pargyline and deprenyl appear to be more effective in inhibiting pigment formation from tryptamine than serotonin, while in the presence of clorgyline and Lilly 51641, pigment formation from serotonin was preferentially inhibited. Reducing agents like ascorbic acid, cysteine and glutathione were found to block pigment formation significantly. Also, a reduction of pigment formation was noted in the presence of NADH and ethanol but not in the presence of NAD. It was observed that the amount of indole-3-acetaldehyde produced enzymatically from tryptamine under the present experimental conditions is not sufficient to account for the total amount of pigment formed in the standard incubation mixture and the generation of nascent aldehyde has greater contribution in pigment formation than that supplemented to the system exogenously. It appears that indole-3-acetaldehyde, tryptamine and MAO are associated with the process of pigment formation.
研究了在豚鼠肝线粒体存在的情况下，吲哚 - 3 - 乙醛在色胺体外色素形成过程中的代谢意义。在所使用的四种类型的选择性单胺氧化酶（MAO）抑制剂中，优降宁和司来吉兰在抑制色胺形成色素方面似乎比血清素更有效，而在氯吉兰和礼来51641存在的情况下，血清素形成色素的过程则优先受到抑制。发现抗坏血酸、半胱氨酸和谷胱甘肽等还原剂能显著阻止色素形成。此外，在存在NADH和乙醇的情况下色素形成减少，但在存在NAD时则不然。据观察，在当前实验条件下，由色胺酶促产生的吲哚 - 3 - 乙醛的量不足以解释标准孵育混合物中形成的色素总量，并且新生醛的产生在色素形成中比外源添加到系统中的醛的贡献更大。吲哚 - 3 - 乙醛、色胺和MAO似乎与色素形成过程有关。
