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1
Absolute measurement of enhanced fluctuations in assemblies of biomolecules by ultrasonic techniques.通过超声技术对生物分子组装体中增强波动的绝对测量。
Biophys J. 1985 Jun;47(6):751-6. doi: 10.1016/S0006-3495(85)83977-1.
2
Ultrasonic absorption in tobacco mosaic virus and its protein aggregates.
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3
Ultrasonic absorption evidence for enhanced volume fluctuations in the tobacco mosaic virus protein helical aggregate.超声吸收证据表明烟草花叶病毒蛋白螺旋聚集物中的体积涨落增强。
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Normal modes of symmetric protein assemblies. Application to the tobacco mosaic virus protein disk.对称蛋白质聚集体的正常模式。应用于烟草花叶病毒蛋白质盘。
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A theorem on amplitudes of thermal atomic fluctuations in large molecules assuming specific conformations calculated by normal mode analysis.一个关于假设通过简正模式分析计算出的特定构象的大分子中热原子涨落振幅的定理。
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[Propagation velocity of ultrasonic waves in aqueous solutions of nucleic acids (state of water near macromolecules)].[超声波在核酸水溶液中的传播速度(大分子附近的水状态)]
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Ultrasonic absorption evidence for enhanced volume fluctuations in the tobacco mosaic virus protein helical aggregate.超声吸收证据表明烟草花叶病毒蛋白螺旋聚集物中的体积涨落增强。
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3
Entropic elastic processes in protein mechanisms. II. Simple (passive) and coupled (active) development of elastic forces.蛋白质机制中的熵弹性过程。II. 弹力的简单(被动)和耦合(主动)发展
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Entropic elastic processes in protein mechanisms. I. Elastic structure due to an inverse temperature transition and elasticity due to internal chain dynamics.蛋白质机制中的熵弹性过程。I. 由逆温度转变引起的弹性结构及由内部链动力学引起的弹性
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Ultrasonic Cole-Cole diagram for solutions and application to alpha-chymotrypsin.溶液的超声科尔-科尔图及其在α-糜蛋白酶中的应用。
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本文引用的文献

1
Ultrasonic absorption evidence for structural fluctuations in frog virus 3 and its subparticles.蛙病毒3及其亚颗粒结构波动的超声吸收证据
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3981-5. doi: 10.1073/pnas.80.13.3981.
2
[A simple temperature range method in the seconds to hours range and the reversible denaturation of chymotrypsin].
Eur J Biochem. 1968 Apr;4(3):373-7. doi: 10.1111/j.1432-1033.1968.tb00221.x.
3
Chemical relaxation of co-operative conformational transitions of linear biopolymers.
J Theor Biol. 1972 Sep;36(3):569-80. doi: 10.1016/0022-5193(72)90009-4.
4
[The transconformations of a molecule with 3 states].[具有三种状态的分子的构象转变]
C R Acad Hebd Seances Acad Sci D. 1970 Dec 21;271(25):2403-5.
5
[Kinetics of the denaturation of alpha chymotrypsin].[α-胰凝乳蛋白酶变性的动力学]
C R Acad Hebd Seances Acad Sci D. 1971 Feb 1;272(5):747-9.
6
Ultrasonic absorption in tobacco mosaic virus and its protein aggregates.
J Mol Biol. 1985 Jan 5;181(1):103-10. doi: 10.1016/0022-2836(85)90328-6.
7
Temperature-dependence of the kinetics of folding of chymotrypsinogen A.胰凝乳蛋白酶原A折叠动力学的温度依赖性
FEBS Lett. 1976 Jun 15;65(3):293-6. doi: 10.1016/0014-5793(76)80132-9.
8
Ultrasonic absorption evidence of structural fluctuations in viral capsids.病毒衣壳结构波动的超声吸收证据。
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1780-2. doi: 10.1073/pnas.76.4.1780.

通过超声技术对生物分子组装体中增强波动的绝对测量。

Absolute measurement of enhanced fluctuations in assemblies of biomolecules by ultrasonic techniques.

作者信息

Cerf R

出版信息

Biophys J. 1985 Jun;47(6):751-6. doi: 10.1016/S0006-3495(85)83977-1.

DOI:10.1016/S0006-3495(85)83977-1
PMID:4016196
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1435159/
Abstract

By expressing the fluctuation-dissipation theorem explicitly, equations are obtained for the ultrasonic relaxation amplitudes that contain one single molecular parameter, i.e., the fluctuation, or the sum of fluctuations. The absolute measurement of this parameter is therefore possible. The equations apply to a two-state system, to a multistate system and to a linear Ising chain as well. In an aqueous medium, where molar volume changes are important, the ultrasonic relaxation amplitudes are proportional to the volume fluctuations. For assemblies of biomolecules that exhibit enhanced ultrasonic absorption on assembly it is possible to measure the increase on assembly of the sum of fluctuations. In view of application to tobacco mosaic virus protein aggregates, examples are given in which the fluctuations associated with two normal modes of relaxation are equally enhanced when the difference of conformational stability of the states is reduced. The corresponding observable changes of the ultrasonic spectra are described.

摘要

通过明确表达涨落耗散定理,得到了包含单个分子参数(即涨落或涨落之和)的超声弛豫振幅方程。因此,对该参数进行绝对测量是可能的。这些方程同样适用于二态系统、多态系统以及线性伊辛链。在水介质中,摩尔体积变化很重要,超声弛豫振幅与体积涨落成正比。对于在组装时表现出增强超声吸收的生物分子聚集体,可以测量组装时涨落之和的增加量。鉴于其在烟草花叶病毒蛋白聚集体中的应用,给出了一些例子,其中当状态的构象稳定性差异减小时,与两种正常弛豫模式相关的涨落会同等增强。描述了超声光谱相应的可观测变化。