He Ying, Zhang Bo, Xin Yu, Wang Wenxiu, Wang Xue, Liu Zhuo, She Yongbo, Guo Rui, Jia Gengjie, Wu Shan, Liu Zhigang
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
Research and Development Center, Xi'an Yinqiao Dairy (Group) Co., Ltd, Xi'an, Shaanxi 710075, China.
Food Funct. 2025 Mar 31;16(7):2703-2717. doi: 10.1039/d4fo06234e.
Autism spectrum disorder (ASD) is a group of heterogeneous neurodevelopmental disorders characterized by social deficits and repetitive behaviors. Increasing evidence suggests that the gut microbiota influences neurodevelopment and behavior. In this study, we established an ASD model by administering valproic acid (VPA) to pregnant females, with male offspring receiving a daily synbiotic intervention for four weeks post-weaning. The results indicate that the synbiotic combination of 2'-Fucosyllactose (2'-FL) and () outperformed that of 2'-FL and () in alleviating social deficits, repetitive behaviors, neuronal damage, and dysregulated expression of social-related genes and neuroinflammatory markers in ASD mice. Additionally, the intervention with 2'-FL and improved gut morphology and barrier integrity, reduced gut inflammation, and optimized the gut microbiota structure by increasing the abundance of and , known producers of short-chain fatty acids (SCFAs). Notably, levels of acetate, propionate, and butyrate were significantly elevated in fecal samples. In summary, the synbiotic combination of 2'-FL and supports gut microbiota homeostasis, enhances fecal SCFA levels, and mitigates neurodevelopmental abnormalities in ASD mice.
自闭症谱系障碍（ASD）是一组异质性神经发育障碍，其特征为社交缺陷和重复行为。越来越多的证据表明，肠道微生物群会影响神经发育和行为。在本研究中，我们通过给怀孕雌性小鼠施用丙戊酸（VPA）建立了一个ASD模型，雄性后代在断奶后接受为期四周的每日合生元干预。结果表明，2'-岩藻糖基乳糖（2'-FL）与（此处原文缺失内容）的合生元组合在减轻ASD小鼠的社交缺陷、重复行为、神经元损伤以及社交相关基因和神经炎症标志物的表达失调方面，优于2'-FL与（此处原文缺失内容）的组合。此外，2'-FL与（此处原文缺失内容）的干预改善了肠道形态和屏障完整性，减轻了肠道炎症，并通过增加短链脂肪酸（SCFAs）的已知产生者（此处原文缺失内容）和（此处原文缺失内容）的丰度优化了肠道微生物群结构。值得注意的是，粪便样本中乙酸盐、丙酸盐和丁酸盐的水平显著升高。总之，2'-FL与（此处原文缺失内容）的合生元组合支持肠道微生物群稳态，提高粪便SCFA水平，并减轻ASD小鼠的神经发育异常。
