Boluda-Navarro Mireia
Health in Code (HiC), Valencia, Spain.
Methods Mol Biol. 2025;2914:141-163. doi: 10.1007/978-1-0716-4462-1_12.
The Olink Explore platform enables high-throughput protein biomarker discovery through Proximity Extension Assay (PEA) technology combined with Next Generation Sequencing (NGS) on Illumina instruments. This approach allows for the simultaneous measurement of thousands of human plasma proteins with minimal sample volumes. The Explore 3072 library offers approximately 3000 protein assays, while the smaller Explore 384-plex panels cater to more targeted studies. The platform excels in detecting low-abundance proteins, such as cytokines and chemokines, and is particularly effective for challenging sample types like cerebrospinal fluid (CSF), where protein content is typically low. In this chapter, we emphasize critical dry-lab considerations, including CSF handling, study design, sample size determination, instrumentation requirements, and post-experiment data management. Proper planning and execution of these factors are essential for optimizing performance and ensuring reliable outcomes when using Olink's platform.
Olink Explore平台通过邻近延伸分析（PEA）技术与Illumina仪器上的下一代测序（NGS）相结合，实现高通量蛋白质生物标志物的发现。这种方法能够以最小的样本量同时测量数千种人血浆蛋白。Explore 3072文库提供约3000种蛋白质检测，而较小的Explore 384孔板则适用于更具针对性的研究。该平台在检测低丰度蛋白（如细胞因子和趋化因子）方面表现出色，对于蛋白质含量通常较低的挑战性样本类型（如脑脊液（CSF））尤为有效。在本章中，我们强调关键的实验前考虑因素，包括脑脊液处理、研究设计、样本量确定、仪器要求和实验后数据管理。在使用Olink平台时，对这些因素进行妥善规划和执行对于优化性能和确保可靠结果至关重要。
