Carneiro Andreia Abadia Borges, Patekar Soumitra, Goyal Mansi, de Almeida Samilla, Dayarathne Nethmi Kulanika, De Campos Samanta Bolzan, Bally Julia, Hassanpour Morteza, Zhang Zhanying
School of Mechanical, Medical and Process Engineering, Centre for Agriculture and the Bioeconomy, Queensland University of Technology, 2 George St, Brisbane, Queensland 4000, Australia.
School of Biology and Environmental Science, Centre for Agriculture and the Bioeconomy, Queensland University of Technology, 2 George St, Brisbane, Queensland 4000, Australia.
Int J Biol Macromol. 2025 May;309(Pt 1):142589. doi: 10.1016/j.ijbiomac.2025.142589. Epub 2025 Mar 31.
This study investigates the production of lignin/silica hybrid nanoparticles (LSNPs) from rice husk, an abundant agricultural byproduct, for the delivery of soybean trypsin inhibitor (STI), a bioinsecticide. Lignin was extracted from rice husk under alkaline conditions and co-precipitated with silica to form LSNPs. Characterization revealed that lignin imparted hydrophobicity to the nanoparticles and increased their surface area, enhancing their potential for pesticide delivery. The hybrid nanoparticles were evaluated for their ability to resist washout, control STI release, and provide effective biocontrol against cotton bollworm larvae - one of the most damaging pests in cotton crops. The results indicates that lignin played a critical role in imparting hydrophobicity to the nanoparticles, significantly enhancing their adhesion to hydrophobic plants such as cotton. The hybrid nano-formulations exhibited superior foliar adherence, washout resistance, and pH-responsive release (28.1 % at pH 9). STI delivered with LSNP achieved 99.1 % insect weight reduction, and complete (100 %) mortality rate compared to 75.8 % weight reduction and 66.7 % mortality rate when delivered by pure silica. This work highlights the synergistic potential of combining lignin and silica from the same bio-based source in enhancing both foliar adhesion and bioactivity of biopesticides, offering a promising alternative for sustainable pest management in agriculture.
本研究探索了以丰富的农业副产品稻壳为原料制备木质素/二氧化硅杂化纳米颗粒（LSNPs），用于递送生物杀虫剂大豆胰蛋白酶抑制剂（STI）。在碱性条件下从稻壳中提取木质素，并与二氧化硅共沉淀以形成LSNPs。表征显示，木质素赋予纳米颗粒疏水性并增加其表面积，增强了它们递送农药的潜力。对杂化纳米颗粒抵抗冲刷的能力、控制STI释放的能力以及对棉铃虫幼虫（棉花作物中最具破坏性的害虫之一）提供有效生物防治的能力进行了评估。结果表明，木质素在赋予纳米颗粒疏水性方面发挥了关键作用，显著增强了它们对棉花等疏水植物的附着力。杂化纳米制剂表现出优异的叶面附着力、抗冲刷性和pH响应释放（在pH 9时为28.1%）。与通过纯二氧化硅递送时75.8%的体重减轻和66.7%的死亡率相比，用LSNP递送的STI实现了99.1%的昆虫体重减轻和100%的死亡率。这项工作突出了将来自同一生物基源的木质素和二氧化硅结合起来在增强生物农药的叶面附着力和生物活性方面的协同潜力，为农业可持续害虫管理提供了一个有前景的替代方案。
