• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

缺血性脑卒中患者外周血单个核细胞中miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p簇的表达

Expression of miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p clusters in peripheral blood mononuclear cells of ischemic stroke.

作者信息

Feng Tingting, Wang Lizhen, Zhou Ruijiao, Yu Juming, Wang Shunxian, Deng Xiaodong, Zhang Wei, Wang Binru, Li Fengjiao, Ma Ying

机构信息

Department of Neurology, Affiliated Hospital of North Sichuan Medical College, Sichuan, China.

Department of Neurology, Sichuan Lansheng Brain Hospital, Sichuan, China.

出版信息

Sci Rep. 2025 Apr 1;15(1):11194. doi: 10.1038/s41598-025-86841-y.

DOI:10.1038/s41598-025-86841-y
PMID:40169609
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11961610/
Abstract

To estimate the correlation between miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p clusters and ischemic stroke (IS). 83 hospitalized patients diagnosed with IS (experimental group) and 50 healthy subjects (control group) were enrolled in the Affiliated Hospital of North Sichuan Medical College from July 2020 to December 2020. The levels of miRNA-338-3p, miRNA-1250-5p, and miRNA-3065-5p in peripheral blood mononuclear cells (PBMCs) were measured by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR). The expressions of miRNA-1250-5p and miRNA-3065-5p were significantly higher in the experimental group compared to the control group (2.04 ± 0.22 vs. 1.54 ± 0.33, P = 0.002, 6.41 ± 2.17 vs. 1.42 ± 0.24, P < 0.001, respectively) No significant difference in miRNA-338-3p expression was observed between the experimental and control groups (1.87 ± 0.22 vs. 1.25 ± 0.11, P = 0.309). The expression levels of miRNA-1250-5p increased after 24 h and no more than 7 days of disease progression but decreased after 7 days compared to baseline (P < 0.05). The expression levels of miRNA-3065-5p and miRNA-338-3p in patients with a discharge National Institutes of Health Stroke Scale (NIHSS) score greater than 33 were higher than those in the group with a score of 3 or less (P < 0.05). Additionally, the expression level of miRNA-3065-5p in patients with discharged mRS scores of 3 or higher was greater than in patients with discharged mRS scores of 2 or lower (P < 0.05). The miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p clusters showed a positive correlation with neutrophil percentage and a negative correlation with lymphocyte percentage (P < 0.05). Furthermore, miRNA-338-3p, miRNA-1250-5p, and miRNA-3065-5p significantly correlated in IS (P < 0.001). miRNA-1250-5p and miRNA-3065-5p may be associated with IS.

摘要

评估miRNA - 338 - 3p/miRNA - 1250 - 5p/miRNA - 3065 - 5p簇与缺血性卒中（IS）之间的相关性。2020年7月至2020年12月，在川北医学院附属医院纳入了83例确诊为IS的住院患者（实验组）和50例健康受试者（对照组）。采用实时定量逆转录聚合酶链反应（RT - qPCR）检测外周血单个核细胞（PBMCs）中miRNA - 338 - 3p、miRNA - 1250 - 5p和miRNA - 3065 - 5p的水平。与对照组相比，实验组中miRNA - 1250 - 5p和miRNA - 3065 - 5p的表达显著更高（分别为2.04 ± 0.22对1.54 ± 0.33，P = 0.002；6.41 ± 2.17对1.42 ± 0.24，P < 0.001）。实验组和对照组之间miRNA - 338 - 3p表达无显著差异（1.87 ± 0.22对1.25 ± 0.11，P = 0.309）。miRNA - 1250 - 5p的表达水平在疾病进展24小时后至不超过7天升高，但与基线相比7天后降低（P < 0.05）。出院时美国国立卫生研究院卒中量表（NIHSS）评分大于33分的患者中miRNA - 3065 - 5p和miRNA - 338 - 3p的表达水平高于评分3分及以下的组（P < 0.05）。此外，出院时改良Rankin量表（mRS）评分3分及以上的患者中miRNA - 3065 - 5p的表达水平高于mRS评分2分及以下的患者（P < 0.05）。miRNA - 338 - 3p/miRNA - 1250 - 5p/miRNA - 3065 - 5p簇与中性粒细胞百分比呈正相关，与淋巴细胞百分比呈负相关（P < 0.05）。此外，miRNA - 338 - 3p、miRNA - 1250 - 5p和miRNA - 3065 - 5p在IS中显著相关（P < 0.001）。miRNA - 1250 - 5p和miRNA - 3065 - 5p可能与IS相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c36f/11961610/5d7f51b038c0/41598_2025_86841_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c36f/11961610/abe626a5da91/41598_2025_86841_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c36f/11961610/a58524b5afd9/41598_2025_86841_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c36f/11961610/fbd04f56d1a4/41598_2025_86841_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c36f/11961610/83444e22885b/41598_2025_86841_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c36f/11961610/d6070b318f67/41598_2025_86841_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c36f/11961610/5d7f51b038c0/41598_2025_86841_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c36f/11961610/abe626a5da91/41598_2025_86841_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c36f/11961610/a58524b5afd9/41598_2025_86841_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c36f/11961610/fbd04f56d1a4/41598_2025_86841_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c36f/11961610/83444e22885b/41598_2025_86841_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c36f/11961610/d6070b318f67/41598_2025_86841_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c36f/11961610/5d7f51b038c0/41598_2025_86841_Fig6_HTML.jpg

相似文献

1
Expression of miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p clusters in peripheral blood mononuclear cells of ischemic stroke.缺血性脑卒中患者外周血单个核细胞中miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p簇的表达
Sci Rep. 2025 Apr 1;15(1):11194. doi: 10.1038/s41598-025-86841-y.
2
The Diagnostic Value of Serum miRNA-221-3p, miRNA-382-5p, and miRNA-4271 in Ischemic Stroke.血清miRNA-221-3p、miRNA-382-5p和miRNA-4271在缺血性卒中中的诊断价值
J Stroke Cerebrovasc Dis. 2017 May;26(5):1055-1060. doi: 10.1016/j.jstrokecerebrovasdis.2016.12.019. Epub 2017 Jan 19.
3
RNA-Seq Identifies Circulating miR-125a-5p, miR-125b-5p, and miR-143-3p as Potential Biomarkers for Acute Ischemic Stroke.RNA-Seq 鉴定出循环 miR-125a-5p、miR-125b-5p 和 miR-143-3p 可作为急性缺血性脑卒中的潜在生物标志物。
Circ Res. 2017 Sep 29;121(8):970-980. doi: 10.1161/CIRCRESAHA.117.311572. Epub 2017 Jul 19.
4
The potential role of micro-RNA 125b-5p level in predicting outcome from thrombolytic therapy in patients with acute ischemic stroke.微小 RNA 125b-5p 水平在预测急性缺血性脑卒中患者溶栓治疗预后中的作用。
J Thromb Thrombolysis. 2023 Aug;56(2):275-282. doi: 10.1007/s11239-023-02831-9. Epub 2023 Jun 8.
5
Differentially expressed microRNAs in peripheral blood mononuclear cells of non-segmental vitiligo and their clinical significance.非节段性白癜风患者外周血单个核细胞中差异表达的 microRNAs 及其临床意义。
J Clin Lab Anal. 2021 Feb;35(2):e23648. doi: 10.1002/jcla.23648. Epub 2020 Nov 10.
6
Increased Expression of mir-34a-5p and Clinical Association in Acute Ischemic Stroke Patients and in a Rat Model.急性缺血性中风患者及大鼠模型中mir-34a-5p表达增加及其临床关联
Med Sci Monit. 2016 Aug 22;22:2950-5. doi: 10.12659/msm.900237.
7
Plasma microRNAs as potential new biomarkers for early detection of early gastric cancer.血浆 microRNAs 作为早期胃癌早期检测的潜在新型生物标志物。
World J Gastroenterol. 2019 Apr 7;25(13):1580-1591. doi: 10.3748/wjg.v25.i13.1580.
8
Expression Profile of miRNA-17-3p and miRNA-17-5p Genes in Gastric Cancer Patients with Helicobacter pylori Infection.幽门螺杆菌感染的胃癌患者中miRNA - 17 - 3p和miRNA - 17 - 5p基因的表达谱
J Gastrointest Cancer. 2021 Mar;52(1):130-137. doi: 10.1007/s12029-019-00319-5.
9
Identification of plasma miR-4505, miR-4743-5p and miR-4750-3p as novel diagnostic biomarkers for coronary artery disease in patients with type 2 diabetes mellitus: a case-control study.鉴定血浆 miR-4505、miR-4743-5p 和 miR-4750-3p 作为 2 型糖尿病患者冠心病的新型诊断生物标志物：病例对照研究。
Cardiovasc Diabetol. 2024 Jul 29;23(1):278. doi: 10.1186/s12933-024-02374-0.
10
PBMCs to Stress-Associated miR-18a-5p and miR-22-3p Ratios as New Indicators of Metabolic Syndrome.外周血单个核细胞应激相关 miR-18a-5p 和 miR-22-3p 比值作为代谢综合征新的标志物。
Biomed Res Int. 2020 Apr 24;2020:8159342. doi: 10.1155/2020/8159342. eCollection 2020.

引用本文的文献

1
MiR338-3p expression in extracellular vesicles after severe trauma with or without traumatic brain injury.伴有或不伴有创伤性脑损伤的严重创伤后细胞外囊泡中MiR338 - 3p的表达。
Brain Commun. 2025 Jun 21;7(4):fcaf242. doi: 10.1093/braincomms/fcaf242. eCollection 2025.

本文引用的文献

1
RVD2 emerges as a serological marker in relation to severity and six-month clinical outcome following acute intracerebral hemorrhage: A prospective cohort study from a single academic institution.RVD2 作为一种与急性脑出血严重程度和 6 个月临床结局相关的血清学标志物出现：来自单一学术机构的前瞻性队列研究。
Clin Chim Acta. 2025 Jan 15;565:119988. doi: 10.1016/j.cca.2024.119988. Epub 2024 Oct 3.
2
Efficacy and safety of Ginkgolide with intravenous alteplase thrombolysis in acute ischemic stroke with large vessel occlusion: a subgroup analysis of GIANT.银杏内酯联合静脉注射阿替普酶溶栓治疗急性大血管闭塞性缺血性卒中的疗效和安全性：GIANT研究的亚组分析
Front Pharmacol. 2024 Aug 30;15:1452174. doi: 10.3389/fphar.2024.1452174. eCollection 2024.
3
Safety and efficacy of tirofiban in preventing neurological deterioration in acute ischemic stroke (TREND): Protocol for an investigator-initiated, multicenter, prospective, randomized, open-label, masked endpoint trial.替罗非班预防急性缺血性卒中神经功能恶化的安全性和有效性（TREND）：一项研究者发起的多中心、前瞻性、随机、开放标签、盲法终点试验方案
Brain Circ. 2024 Jun 26;10(2):168-173. doi: 10.4103/bc.bc_93_23. eCollection 2024 Apr-Jun.
4
Long-term outcomes among ischemic stroke TOAST subtypes: A 12-year Cohort study in China.中国一项为期 12 年的队列研究：缺血性卒中 TOAST 亚型的长期结局。
J Stroke Cerebrovasc Dis. 2024 Aug;33(8):107783. doi: 10.1016/j.jstrokecerebrovasdis.2024.107783. Epub 2024 Jun 18.
5
Deciphering the Role of microRNAs: Unveiling Clinical Biomarkers and Therapeutic Avenues in Atrial Fibrillation and Associated Stroke-A Systematic Review.解析 microRNAs 的作用：揭示心房颤动及相关卒中的临床生物标志物和治疗新途径——系统综述。
Int J Mol Sci. 2024 May 20;25(10):5568. doi: 10.3390/ijms25105568.
6
Prognostic significance of international normalised ratio and prothrombin time in Chinese acute ischaemic stroke patients.国际标准化比值和凝血酶原时间对中国急性缺血性脑卒中患者的预后意义。
Postgrad Med J. 2023 May 22;99(1170):333-339. doi: 10.1136/postgradmedj-2021-141204.
7
In vitro validation of an in vivo phenotyping drug cocktail for major drug transporters in humans.在体药物表型鸡尾酒在人体主要药物转运体中的体内验证。
Eur J Pharm Sci. 2023 Jul 1;186:106459. doi: 10.1016/j.ejps.2023.106459. Epub 2023 May 3.
8
Circulating Extracellular-Vesicle-Incorporated MicroRNAs as Potential Biomarkers for Ischemic Stroke in Patients With Cancer.循环细胞外囊泡包裹的微小RNA作为癌症患者缺血性中风的潜在生物标志物
J Stroke. 2023 May;25(2):251-265. doi: 10.5853/jos.2022.02327. Epub 2023 May 2.
9
Anti-CHAC1 exosomes for nose-to-brain delivery of miR-760-3p in cerebral ischemia/reperfusion injury mice inhibiting neuron ferroptosis.用于在脑缺血/再灌注损伤小鼠中鼻脑递送miR-760-3p以抑制神经元铁死亡的抗CHAC1外泌体。
J Nanobiotechnology. 2023 Mar 27;21(1):109. doi: 10.1186/s12951-023-01862-x.
10
miR-671-5p Upregulation Attenuates Blood-Brain Barrier Disruption in the Ischemia Stroke Model Via the NF-кB/MMP-9 Signaling Pathway.miR-671-5p 通过 NF-кB/MMP-9 信号通路上调减轻缺血性脑卒中模型中的血脑屏障破坏。
Mol Neurobiol. 2023 Jul;60(7):3824-3838. doi: 10.1007/s12035-023-03318-7. Epub 2023 Mar 22.