Expression of miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p clusters in peripheral blood mononuclear cells of ischemic stroke.

To estimate the correlation between miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p clusters and ischemic stroke (IS). 83 hospitalized patients diagnosed with IS (experimental group) and 50 healthy subjects (control group) were enrolled in the Affiliated Hospital of North Sichuan Medical College from July 2020 to December 2020. The levels of miRNA-338-3p, miRNA-1250-5p, and miRNA-3065-5p in peripheral blood mononuclear cells (PBMCs) were measured by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR). The expressions of miRNA-1250-5p and miRNA-3065-5p were significantly higher in the experimental group compared to the control group (2.04 ± 0.22 vs. 1.54 ± 0.33, P = 0.002, 6.41 ± 2.17 vs. 1.42 ± 0.24, P < 0.001, respectively) No significant difference in miRNA-338-3p expression was observed between the experimental and control groups (1.87 ± 0.22 vs. 1.25 ± 0.11, P = 0.309). The expression levels of miRNA-1250-5p increased after 24 h and no more than 7 days of disease progression but decreased after 7 days compared to baseline (P < 0.05). The expression levels of miRNA-3065-5p and miRNA-338-3p in patients with a discharge National Institutes of Health Stroke Scale (NIHSS) score greater than 33 were higher than those in the group with a score of 3 or less (P < 0.05). Additionally, the expression level of miRNA-3065-5p in patients with discharged mRS scores of 3 or higher was greater than in patients with discharged mRS scores of 2 or lower (P < 0.05). The miRNA-338-3p/miRNA-1250-5p/miRNA-3065-5p clusters showed a positive correlation with neutrophil percentage and a negative correlation with lymphocyte percentage (P < 0.05). Furthermore, miRNA-338-3p, miRNA-1250-5p, and miRNA-3065-5p significantly correlated in IS (P < 0.001). miRNA-1250-5p and miRNA-3065-5p may be associated with IS.