盘基网柄菌的腺苷酸环化酶：溶解与锰离子依赖性
Adenylate cyclase of Dictyostelium discoideum: solubilization and Mn2+-dependency.
作者信息
Hagmann J
出版信息
Cell Biol Int Rep. 1985 May;9(5):491-4. doi: 10.1016/0309-1651(85)90157-2.
PMID:4016966
Abstract
Adenylate cyclase from Dictyostelium discoideum was solubilized under alkaline conditions using the zwitterionic detergent CHAPS. In contrast to the membrane bound adenylate cyclase, the solubilized enzyme can use only Mn2+, but is inactive in the presence of Mg2+.
摘要
使用两性离子去污剂CHAPS在碱性条件下溶解盘基网柄菌的腺苷酸环化酶。与膜结合的腺苷酸环化酶不同，溶解的酶仅能利用Mn2+，而在Mg2+存在时无活性。
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