Jiang Lingling, Wang Pu, Zhang Gang, Niu Xiaoxia, Liu Qiang, Liang Ruijin, Zhang Sinong, Li Yong
School of Life Sciences, Ningxia University, Yinchuan, China.
China Medical University, Shenyang, China.
BMC Vet Res. 2025 Apr 2;21(1):232. doi: 10.1186/s12917-025-04640-z.
Bovine diarrhea virus (BVDV) is considered to be the most common pathogen causing severe diarrhea in cattle worldwide and can cause Bovine viral diarrhea (BVD). Clinical manifestations of fever, diarrhea, ulcers, and abortions, resulting in significant economic losses to the cattle industry. The development of an efficient, rapid and sensitive assay suitable for field conditions is of great significance for its early detection. Recombinase polymerase amplification (RPA) is a novel nucleic acid amplification method that has been widely used in the diagnosis of infectious diseases.
We developed a rapid assay (RPAS) combining RPA with SYBR Green I for the detection of BVDV. The BVDV RPAS assay was performed at 37 °C in 25 min. The minimum detection limit of the RPAS assay is 1 × 10 copies/µL in sunlight and 1 × 10 copies/µL in ultraviolet light, and there is no cross-reactivity with other viruses that cause gastrointestinal and respiratory infections in cattle. The coincidence rate of BVDV RPAS in clinical samples was higher than that of PCR.
The BVDV RPAS assay established in this study has high sensitivity and specificity, and is expected to be a powerful tool for the prevention and control of BVD.
牛病毒性腹泻病毒（BVDV）被认为是全球范围内引起牛严重腹泻的最常见病原体，可导致牛病毒性腹泻（BVD）。临床表现为发热、腹泻、溃疡和流产，给养牛业造成重大经济损失。开发一种适用于现场条件的高效、快速且灵敏的检测方法对其早期检测具有重要意义。重组酶聚合酶扩增（RPA）是一种新型核酸扩增方法，已广泛应用于传染病诊断。
我们开发了一种将RPA与SYBR Green I相结合用于检测BVDV的快速检测方法（RPAS）。BVDV RPAS检测在37℃下进行25分钟。RPAS检测在日光下的最低检测限为1×10拷贝/微升，在紫外光下为1×10拷贝/微升，并且与其他引起牛胃肠道和呼吸道感染的病毒无交叉反应。BVDV RPAS在临床样本中的符合率高于PCR。
本研究建立的BVDV RPAS检测方法具有高灵敏度和特异性，有望成为BVD防控的有力工具。
