McPherson R A, Brown K D, Agarwal R P, Threatte G A, Jacobson R J
Clin Chem. 1985 Aug;31(8):1355-7.
Measured triglyceride concentrations were extremely low (less than 100 mg/L) in the serum of some patients who were receiving hydroxyurea for myeloproliferative diseases. The assay being used to quantify triglycerides was a "cascaded" enzymatic method involving (a) lipase, to generate glycerol from triglycerides; (b) glycerol oxidase, to convert glycerol to glyceraldehyde, with generation of hydrogen peroxide; and (c) peroxidase, which acts on the hydrogen peroxide with subsequent coupled generation of a red-violet quinone (reagent system used in the Technicon RA-1000). Hydroxyurea added to serum samples appeared to inhibit the action of glycerol oxidase, with a stoichiometric relation to the concentration of substrate (a decrease of roughly 2.4 mmol/L in measured triglyceride per 1 mmol of hydroxyurea per liter). A different enzymatic assay for triglycerides, which involves glycerol kinase (Beckman Instruments) did not show this effect of hydroxyurea.
在一些接受羟基脲治疗骨髓增殖性疾病的患者血清中，测得的甘油三酯浓度极低（低于100mg/L）。用于定量甘油三酯的检测方法是一种“级联”酶法，包括：（a）脂肪酶，从甘油三酯生成甘油；（b）甘油氧化酶，将甘油转化为甘油醛，并生成过氧化氢；（c）过氧化物酶，作用于过氧化氢，随后偶联生成红紫色醌（Technicon RA - 1000中使用的试剂系统）。添加到血清样本中的羟基脲似乎抑制了甘油氧化酶的作用，与底物浓度呈化学计量关系（每升每1mmol羟基脲，测得的甘油三酯大约降低2.4mmol/L）。另一种涉及甘油激酶的甘油三酯酶法检测（贝克曼仪器公司）未显示羟基脲的这种作用。
