Zhuang Linlin, Gong Jiansen, Zhang Di, Zhang Ping, Zhao Ying, Yang Jianbo, Sun Li, Zhang Yu, Shen Qiuping
School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, People's Republic of China.
State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering and Basic Medicine Research and Innovation Center of Ministry of Education, Zhongda Hospital, Southeast University, Nanjing, 211102, People's Republic of China.
Discov Nano. 2025 Apr 2;20(1):65. doi: 10.1186/s11671-025-04237-3.
This paper provides a comprehensive overview of the diverse applications and innovations of nanoparticles in the detection of Salmonella. It encompasses a comprehensive range of novel methods, including efficient enrichment, nucleic acid extraction, immunoassays, nucleic acid tests, biosensors, and emerging strategies with the potential for future applications. The surface modification of specific antibodies or ligands enables nanoparticles to achieve highly selective capture of Salmonella, while optimizing the nucleic acid extraction process and improving detection efficiency. The employment of nanoparticles in immunological and nucleic acid tests markedly enhances the specificity and sensitivity of the reaction, thereby optimizing the determination of detection results. Moreover, the distinctive physicochemical properties of nanoparticles enhance the sensitivity, selectivity, and stability of biosensors, thereby facilitating the rapid advancement of bio-detection technologies. It is particularly noteworthy that there has been significant advancement in the application and innovative research of nanozymes in molecular assays. This progress has not only resulted in enhanced detection efficiency but has also facilitated innovation and improvement in detection technologies. As nanotechnologies continue to advance, the use of metal and metal oxide nanoparticles in Salmonella detection is likely to become a more promising and reliable strategy for ensuring food safety and public health.
本文全面概述了纳米颗粒在沙门氏菌检测中的多种应用和创新。它涵盖了一系列新颖的方法，包括高效富集、核酸提取、免疫测定、核酸检测、生物传感器以及具有未来应用潜力的新兴策略。特定抗体或配体的表面修饰使纳米颗粒能够实现对沙门氏菌的高度选择性捕获，同时优化核酸提取过程并提高检测效率。纳米颗粒在免疫和核酸检测中的应用显著提高了反应的特异性和灵敏度，从而优化了检测结果的判定。此外，纳米颗粒独特的物理化学性质增强了生物传感器的灵敏度、选择性和稳定性，从而推动了生物检测技术的快速发展。特别值得注意的是，纳米酶在分子检测中的应用和创新研究取得了重大进展。这一进展不仅提高了检测效率，还促进了检测技术的创新和改进。随着纳米技术的不断进步，金属和金属氧化物纳米颗粒在沙门氏菌检测中的应用可能会成为确保食品安全和公共卫生的更有前景和可靠的策略。
