Nair R M, Menon M P, Sagel J
Clin Physiol Biochem. 1985;3(4):157-65.
Lactate dehydrogenase (LDH) isoenzyme profiles in human platelets and the sera of patients with type I and II diabetes mellitus and vascular complications, as well as normal subjects were measured utilizing a recently established, modified micromethod. LDH-3 was the predominating species in platelets (37.5 +/- 3.0%), with LDH-2, 1, 4 and 5 following in decreasing order of concentration. The LDH-3/LDH-4 ratio in platelets varied from 6.2 to 1.38. Type I and type II diabetic patients with vascular complications showed a significantly higher ratio for LDH-3/LDH-4 (3.99 +/- 1.20 for DM I, 2.16 +/- 0.25 for DM II patients) than the mean ratio for normal subjects (1.14 +/- 0.08). This platelet-specific LDH isoenzyme pattern may be the result of frequent in vivo platelet-vessel wall interactions in the diabetic patients whose platelets are known to be hyperaggregable in in vitro test systems. Since non-diabetic patients patients with vascular complications also displayed a similarly elevated LDH-3/LDH-4 ratio, a wider classification is preferable, although the measurement of the LDH isoenzyme pattern will be helpful in assessing diabetic vascular complications.
利用最近建立的改良微量法，测定了人类血小板以及Ⅰ型和Ⅱ型糖尿病伴血管并发症患者及正常受试者血清中的乳酸脱氢酶（LDH）同工酶谱。LDH-3是血小板中的主要类型（37.5±3.0%），LDH-2、1、4和5的浓度依次递减。血小板中LDH-3/LDH-4的比值在6.2至1.38之间变化。患有血管并发症的Ⅰ型和Ⅱ型糖尿病患者的LDH-3/LDH-4比值（Ⅰ型糖尿病患者为3.99±1.20，Ⅱ型糖尿病患者为2.16±0.25）显著高于正常受试者的平均比值（1.14±0.08）。这种血小板特异性的LDH同工酶模式可能是由于糖尿病患者体内血小板与血管壁频繁相互作用的结果，已知糖尿病患者的血小板在体外测试系统中具有高聚集性。由于患有血管并发症的非糖尿病患者也表现出类似升高的LDH-3/LDH-4比值，因此尽管LDH同工酶谱的测定有助于评估糖尿病血管并发症，但更广泛的分类可能更可取。
