Zarzycka Marta, Korzekwa Anna Justyna, Dulińska-Litewka Joanna, Kaingu Catherine Kaluwa, Kotula-Balak Małgorzata
Chair of Medical Biochemistry, Jagiellonian University Medical College, Kopernika 7, 31-034, Kraków, Poland.
Research Team of Biodiversity Protection, Institute of Animal, Reproduction and Food Research of Polish Academy of Sciences (IAR&FR PAS), Tuwima 10 Str., 10-748, Olsztyn, Poland.
Histochem Cell Biol. 2025 Apr 2;163(1):41. doi: 10.1007/s00418-025-02373-6.
Prostate cancer is a frequently diagnosed cancer in men, but today the prognosis of local cancer and its metastasis has improved markedly owing to multiple treatment options. In therapy, various molecular entities that induce the reversion of malignancy with the restoration of mature cells of the same histological lineage are still under development. The antler of the red deer is a large and easily available source of stem cells, rich in products with promising applications in cancer treatments. Here, for the first time, antler stem cell culture medium was used for the treatment of prostate cancer cells in vitro to determine the cellular and molecular effects. Our data revealed that antler stem cell culture medium promotes pronounced changes in the expression of the vaspin receptor GRP78, intercellular adhesion molecule 1 (ICAM-1), androgen receptor (AR), leptin, vaspin, and SRC and AKT kinase signaling pathways (connected with autophagy and cell cycle inhibition and apoptosis induction). Moreover, the inhibition of cell migration was also revealed. These results indicate useful properties of red deer antler stem cell culture medium that can control molecular and cellular mechanisms that halt carcinogenesis. All these can be considered targets for further cancer stem cell treatment or cancer diagnostic improvements.
前列腺癌是男性中常被诊断出的癌症，但如今，由于多种治疗选择，局部癌症及其转移的预后已显著改善。在治疗方面，各种能诱导恶性肿瘤逆转并恢复相同组织学谱系成熟细胞的分子实体仍在研发中。马鹿的鹿角是干细胞的一个丰富且易于获取的来源，富含在癌症治疗中具有潜在应用价值的产物。在此，首次使用鹿角干细胞培养基在体外处理前列腺癌细胞，以确定其细胞和分子效应。我们的数据显示，鹿角干细胞培养基能促使血管丝氨酸蛋白酶抑制剂受体GRP78、细胞间黏附分子1（ICAM - 1）、雄激素受体（AR）、瘦素、血管丝氨酸蛋白酶抑制剂、SRC和AKT激酶信号通路（与自噬、细胞周期抑制和凋亡诱导相关）的表达发生显著变化。此外，还发现细胞迁移受到抑制。这些结果表明马鹿鹿角干细胞培养基具有有益特性，能够控制阻止癌症发生的分子和细胞机制。所有这些都可被视为进一步进行癌症干细胞治疗或改善癌症诊断的靶点。
