Largo-de la Torre Andrea, Ferre Ignacio, Sánchez-Sánchez Roberto, Regidor-Cerrillo Javier, Ortega-Mora Luis Miguel
SALUVET-Innova, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain.
SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain.
Vet Res. 2025 Apr 2;56(1):73. doi: 10.1186/s13567-025-01507-x.
Type II reference isolates of Toxoplasma gondii are widely used in animal toxoplasmosis models, but studies with Type III isolates remain scarce. In addition, these methods often rely on laboratory-adapted parasite stages that may not reflect natural infection. This study presents a new murine model based on an oral infection with oocysts from a recently obtained Type III isolate, TgShSp24, which exhibited remarkable morbidity and a distinct tissue distribution during chronic infection, differing from the recently obtained Type II isolate TgShSp1. This novel model aims to better mimic natural infection and provides a valuable tool for testing drugs and vaccines.
刚地弓形虫II型参考分离株广泛用于动物弓形虫病模型，但关于III型分离株的研究仍然很少。此外，这些方法通常依赖于实验室适应的寄生虫阶段，可能无法反映自然感染情况。本研究提出了一种新的小鼠模型，该模型基于用最近获得的III型分离株TgShSp24的卵囊进行口服感染，在慢性感染期间，该分离株表现出显著的发病率和独特的组织分布，与最近获得的II型分离株TgShSp1不同。这个新模型旨在更好地模拟自然感染，并为测试药物和疫苗提供了一个有价值的工具。
