Bai Pu, Dong Yu
Inner Mongolia Medical University, Hohhot, China.
Ordos School of Clinical Medicine, Inner Mongolia Medical University, Ordos, China.
Front Pharmacol. 2025 Mar 19;16:1547415. doi: 10.3389/fphar.2025.1547415. eCollection 2025.
Sendeng-4 is a Mongolian drug. The Mongolian people have been using it to treat rheumatoid arthritis. At present, an increasing number of Han people are paying attention to the anti-rheumatoid effect of Sendeng-4. However, information on the pharmacokinetics of Sendeng-4 is limited, which limits its wide application in China.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established to study the pharmacokinetics of Sendeng-4.
MS/MS with a negative ionization mode (ESI-) and multiple reaction monitoring at m/z 300.95→193.09 and 317.08→192.10 were detected for (2R, 3R)-dihydromyricetin and myricetin, respectively. The pharmacokinetic parameters were analyzed by DAS 2.0.
The results showed that the plasma concentration time (C-T) curves of (2R, 3R)-dihydromyricetin and myricetin showed double peaks. The T value of (2R, 3R)-dihydromyricetin and myricetin in both groups was 3 h. In absorption, the AUC values of (2R, 3R)-dihydromyricetin and myricetin in the normal group and the arthritis model group were 16.151 ± 2.670 mg·h/L vs. 11.331 ± 0.749 mg·h/L and 2.626 ± 0.400 mg·h/L vs. 2.213 ± 0.388 mg·h/L, respectively. In the distribution, the Vz/F values of (2R, 3R)-dihydromyricetin and myricetin in the normal group and the arthritis model group were 8.212 L/kg vs. 1.744 L/kg and 5.252 L/kg vs. 10.568 L/kg, respectively. In metabolism, the MRT (0-∞) values of (2R, 3R)-dihydromyricetin and myricetin in the normal group and the arthritis model group were 6.848 h vs. 3.476 h and 5.661 h vs. 8.959 h, respectively. In excretion, the CLz/F values of (2R, 3R)-dihydromyricetin and myricetin in the normal group and the arthritis model group were 0.021 vs. 0.024 L/min/kg and 0.018 vs. 0.021 L/min/kg, respectively. There were significant variations in the absorption levels, distribution levels, and elimination rate of (2R, 3R)-dihydromyricetin and myricetin after the administration of Sendeng-4.
The study laid the foundation for the subsequent study of pharmacokinetics of Sendeng-4 in humans. The results of this study will contribute to a better understanding of the activity and clinical application of Sendeng-4 and other related traditional Mongolian drug prescriptions.
森登-4是一种蒙药。蒙古族人民一直用它来治疗类风湿性关节炎。目前，越来越多的汉族人开始关注森登-4的抗类风湿作用。然而，关于森登-4药代动力学的信息有限，这限制了它在中国的广泛应用。
建立液相色谱-串联质谱法（LC-MS/MS）研究森登-4的药代动力学。
采用负离子模式（ESI-）的串联质谱，分别对（2R，3R）-二氢杨梅素和杨梅素进行m/z 300.95→193.09和317.08→192.10的多反应监测。采用DAS 2.0分析药代动力学参数。
结果表明，（2R，3R）-二氢杨梅素和杨梅素的血药浓度-时间（C-T）曲线呈双峰。两组中（2R，3R）-二氢杨梅素和杨梅素的T值均为3小时。吸收方面，正常组和关节炎模型组中（2R，3R）-二氢杨梅素的AUC值分别为16.151±2.670mg·h/L和11.331±0.749mg·h/L，杨梅素的AUC值分别为2.626±0.400mg·h/L和2.213±0.388mg·h/L。分布方面，正常组和关节炎模型组中（2R，3R）-二氢杨梅素的Vz/F值分别为8.212L/kg和1.744L/kg，杨梅素的Vz/F值分别为5.252L/kg和10.568L/kg。代谢方面，正常组和关节炎模型组中（2R，3R）-二氢杨梅素的MRT（0-∞）值分别为6.848小时和3.476小时，杨梅素的MRT（0-∞）值分别为5.661小时和8.959小时。排泄方面，正常组和关节炎模型组中（2R，3R）-二氢杨梅素的CLz/F值分别为0.021和0.024L/min/kg，杨梅素的CLz/F值分别为0.018和0.021L/min/kg。给予森登-4后，（2R，3R）-二氢杨梅素和杨梅素的吸收水平、分布水平和消除率存在显著差异。
该研究为后续森登-4在人体药代动力学研究奠定了基础。本研究结果将有助于更好地理解森登-4及其他相关蒙药传统方剂的活性和临床应用。
