Delamere N A, Williams R N
Exp Eye Res. 1985 Jun;40(6):805-11. doi: 10.1016/0014-4835(85)90125-3.
When the intact rabbit iris-ciliary body was incubated in Tyrode's solution containing 10(-4) M hydrogen peroxide, the concentration of hydrogen peroxide remaining in solution diminished rapidly. The iris-ciliary body was homogenized and centrifuged at 100 000 g. It was observed that the hydrogen peroxide detoxification activity was resident primarily in the 100 000 g supernatant and not in the pellet. The hydrogen peroxide detoxification activity was found to be inactivated by heat and to be non-dialyzable. Gel filtration chromatography experiments revealed that breakdown of hydrogen peroxide by the ciliary body was not a 'bulk effect' due to generalized oxidation of tissue constituents. In fact, the active principle, localized by gel filtration chromatography, identified closely with catalase. These observations indicate that catalase within the iris-ciliary body enables the tissue to detoxify hydrogen peroxide from solution. Catalase might protect the iris-ciliary body from damage by hydrogen peroxide generated by normal physiological or pathophysiological conditions.
当完整的兔虹膜睫状体在含有10⁻⁴M过氧化氢的台氏液中孵育时，溶液中剩余的过氧化氢浓度迅速降低。将虹膜睫状体匀浆并在100000g下离心。观察到过氧化氢解毒活性主要存在于100000g的上清液中，而不存在于沉淀中。发现过氧化氢解毒活性可被热灭活且不可透析。凝胶过滤色谱实验表明，睫状体对过氧化氢的分解不是由于组织成分的普遍氧化导致的“整体效应”。事实上，通过凝胶过滤色谱定位的活性成分与过氧化氢酶密切相关。这些观察结果表明，虹膜睫状体内的过氧化氢酶使组织能够从溶液中解毒过氧化氢。过氧化氢酶可能保护虹膜睫状体免受正常生理或病理生理条件下产生的过氧化氢的损伤。
