通过琼脂糖凝胶等速电泳分析汗液和尿液中的蛋白质。

Analysis of the proteins in sweat and urine by agarose-gel isotachophoresis.

作者信息

Uyttendaele K, De Groote M, Blaton V, Peeters H, Alexander F

出版信息

J Chromatogr. 1977 Feb 11;132(2):261-6. doi: 10.1016/s0021-9673(00)89299-7.

Abstract

A sensitive and practical method is described for the analysis of the proteins contained in human sweat and urine which does not require pre-concentration of the sample. Technical details are provided of the agarose-gel isotachophoresis and the proteinograms of normal and pathological urine samples, as well as proteinograms of human sweat. The method can also be applied as an electro-concentration system in a field-strength gradient. By means of this electro-concentration system, and in combination with immunodiffusion against monospecific antisera, a detection limit of albumin of 50 ng/ml has been obtained.

摘要

本文描述了一种灵敏且实用的方法，用于分析人汗液和尿液中的蛋白质，该方法无需对样品进行预浓缩。文中提供了琼脂糖凝胶等速电泳的技术细节，以及正常和病理尿液样本的蛋白质图谱，还有人汗液的蛋白质图谱。该方法还可作为场强梯度中的电浓缩系统应用。借助此电浓缩系统，并结合针对单特异性抗血清的免疫扩散，已获得白蛋白的检测限为50 ng/ml。