流式细胞仪飞行时间质谱中的样本多路复用：优化单细胞蛋白质组分析的途径。
Sample multiplexing in CyTOF: Path to optimize single-cell proteomic profiling.
作者信息
Muftuoglu Muharrem, Andreeff Michael
机构信息
Section of Molecular Hematology and Therapy, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA.
出版信息
Cell Signal (Middlet). 2024;2(1):113-119. doi: 10.46439/signaling.2.041.
Sample multiplexing significantly enhanced the depth of single-cell proteomic analysis in CyTOF (Cytometry by Time-Of-Flight). New polymer-based chelators have broadened the utility of metal isotopes, enabling improved tagging and simultaneous analysis of multiple samples. These approaches minimize batch effects, streamline experiments, conserve valuable samples, reduce costs, enhance throughput, and increase the accuracy of biological data, thereby facilitating novel discoveries.
样本多路复用显著提高了飞行时间流式细胞术（CyTOF）中单细胞蛋白质组分析的深度。新型基于聚合物的螯合剂拓宽了金属同位素的应用范围，能够实现更好的标记以及多个样本的同时分析。这些方法可将批次效应降至最低，简化实验流程，节省珍贵样本，降低成本，提高通量，并提升生物数据的准确性，从而推动新发现的产生。
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