在十二烷基硫酸钠（SDS）存在的情况下，通过伴刀豆球蛋白A-琼脂糖亲和色谱法在低温下改进糖蛋白的分离。
Improved separation at low temperature of glycoproteins by Con A-Sepharose affinity chromatography in the presence of sodium dodecyl sulfate (SDS).
作者信息
Aono S, Sato H, Semba R, Kashiwamata S
出版信息
Experientia. 1985 Aug 15;41(8):1084-7. doi: 10.1007/BF01952156.
PMID:4018235
Abstract
The Con A-Sepharose affinity chromatography of glycoproteins was even more effective at 4 degrees C than that at room temperature (26-28 degrees C) in the presence of sodium dodecyl sulfate (SDS). Application of this methodology to the separation of several glycoproteins from SDS-solubilized membrane proteins in rat cerebellum, including a glycoprotein characteristic of the Purkinje cells, was successful.
摘要
在存在十二烷基硫酸钠（SDS）的情况下，糖蛋白的伴刀豆球蛋白A-琼脂糖亲和色谱在4℃时比在室温（26 - 28℃）时更有效。将该方法应用于从大鼠小脑的SDS溶解的膜蛋白中分离几种糖蛋白，包括浦肯野细胞特有的一种糖蛋白，获得了成功。
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