• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

致癌性疱疹病毒对人诱导多能干细胞的感染

Infection of human induced pluripotent stem cells by an oncogenic herpesvirus.

作者信息

Fan Jiaojiao, Lin Zhen, Zhang Huiliang, Dai Lu, Qin Zhiqiang

机构信息

Department of Pathology, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR, United States.

Department of Pathology and Laboratory Medicine, Tulane University Health Sciences Center, Tulane Cancer Center, New Orleans, LA, United States.

出版信息

Front Cell Infect Microbiol. 2025 Mar 13;15:1563440. doi: 10.3389/fcimb.2025.1563440. eCollection 2025.

DOI:10.3389/fcimb.2025.1563440
PMID:40182776
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11966036/
Abstract

OBJECTIVE

As one of the major human oncogenic viruses, Kaposi's Sarcoma-associated Herpesvirus (KSHV) is closely related to several cancers such as Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL). KSHV can infect a broad tropism of human primary cells and . Embryonic stem cell-like pluripotent stem cells can be generated by the simultaneous introduction of several factors, into somatic cells, yielding induced pluripotent stem (iPS) cells. However, it remains unclear whether human induced pluripotent stem cells (hiPSCs) are permissive to KSHV and how this oncogenic virus infection may affect cellular gene profile.

METHODS

In the current study, we examined whether hiPSCs were permissive to KSHV infection. The flow cytometry was used to assess the impacts of KSHV infection on hiPSCs viability and apoptosis. The Illumina RNA-Sequencing was used to determine cellular gene profile changed in KSHV-infected hiPSCs and lytically induced cells.

RESULTS

We report that KSHV successfully establishes latent infection in hiPSCs, which can be completely induced to lytic reactivation and release infectious virions. KSHV infection arrests the growth of hiPSCs through inducing cell apoptosis. Transcriptomic analysis revealed significant changes in global cellular gene expression in KSHV-infected hiPSCs as well as lytically induced cells.

CONCLUSION

Our findings demonstrate hiPSCs as a powerful tool to explore the potential impacts of KSHV infection on stem cell functions and virus pathogenesis in stem cell differentiated cells.

摘要

目的

作为主要的人类致癌病毒之一，卡波西肉瘤相关疱疹病毒（KSHV）与卡波西肉瘤（KS）和原发性渗出性淋巴瘤（PEL）等多种癌症密切相关。KSHV能感染多种人类原代细胞。通过向体细胞同时导入几种因子可生成胚胎干细胞样多能干细胞，即诱导多能干细胞（iPS细胞）。然而，人类诱导多能干细胞（hiPSC）是否对KSHV易感以及这种致癌病毒感染如何影响细胞基因谱仍不清楚。

方法

在本研究中，我们检测了hiPSC是否对KSHV感染易感。采用流式细胞术评估KSHV感染对hiPSC活力和凋亡的影响。使用Illumina RNA测序来确定KSHV感染的hiPSC和裂解诱导细胞中细胞基因谱的变化。

结果

我们报告KSHV能在hiPSC中成功建立潜伏感染，且可被完全诱导至裂解再激活并释放感染性病毒粒子。KSHV感染通过诱导细胞凋亡抑制hiPSC的生长。转录组分析显示，KSHV感染的hiPSC以及裂解诱导细胞中整体细胞基因表达有显著变化。

结论

我们的研究结果表明，hiPSC是探索KSHV感染对干细胞功能及病毒在干细胞分化细胞中致病机制潜在影响的有力工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/a3d4d1efc024/fcimb-15-1563440-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/246fd0fe1079/fcimb-15-1563440-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/46bdd7b5f95b/fcimb-15-1563440-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/17ef293eb772/fcimb-15-1563440-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/86a8b5f9c2bb/fcimb-15-1563440-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/e86d139a689d/fcimb-15-1563440-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/363693cf406c/fcimb-15-1563440-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/d5af2cc84fa5/fcimb-15-1563440-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/258cde939747/fcimb-15-1563440-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/a3d4d1efc024/fcimb-15-1563440-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/246fd0fe1079/fcimb-15-1563440-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/46bdd7b5f95b/fcimb-15-1563440-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/17ef293eb772/fcimb-15-1563440-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/86a8b5f9c2bb/fcimb-15-1563440-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/e86d139a689d/fcimb-15-1563440-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/363693cf406c/fcimb-15-1563440-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/d5af2cc84fa5/fcimb-15-1563440-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/258cde939747/fcimb-15-1563440-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ecca/11966036/a3d4d1efc024/fcimb-15-1563440-g009.jpg

相似文献

1
Infection of human induced pluripotent stem cells by an oncogenic herpesvirus.致癌性疱疹病毒对人诱导多能干细胞的感染
Front Cell Infect Microbiol. 2025 Mar 13;15:1563440. doi: 10.3389/fcimb.2025.1563440. eCollection 2025.
2
Repurposing Cytarabine for Treating Primary Effusion Lymphoma by Targeting Kaposi's Sarcoma-Associated Herpesvirus Latent and Lytic Replications.通过靶向卡波氏肉瘤相关疱疹病毒潜伏和裂解复制，重新利用阿糖胞苷治疗原发性渗出性淋巴瘤。
mBio. 2018 May 8;9(3):e00756-18. doi: 10.1128/mBio.00756-18.
3
FoxO1 Suppresses Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication and Controls Viral Latency.FoxO1 抑制卡波氏肉瘤相关疱疹病毒裂解复制并控制病毒潜伏。
J Virol. 2019 Jan 17;93(3). doi: 10.1128/JVI.01681-18. Print 2019 Feb 1.
4
Glycolysis, Glutaminolysis, and Fatty Acid Synthesis Are Required for Distinct Stages of Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication.卡波西肉瘤相关疱疹病毒裂解复制的不同阶段需要糖酵解、谷氨酰胺分解和脂肪酸合成。
J Virol. 2017 Apr 28;91(10). doi: 10.1128/JVI.02237-16. Print 2017 May 15.
5
Glycoproteins gM and gN are indispensable factors for rhesus macaque rhadinovirus replication and spread but can be reconstituted by KSHV chimeras.糖蛋白gM和gN是恒河猴疱疹病毒复制和传播所必需的因子，但可由卡波西肉瘤相关疱疹病毒嵌合体重建。
J Virol. 2025 Mar 18;99(3):e0192224. doi: 10.1128/jvi.01922-24. Epub 2025 Feb 25.
6
KSHV reprograms host RNA splicing via FAM50A to activate STAT3 and drive oncogenic cellular transformation.卡波氏肉瘤相关疱疹病毒通过FAM50A对宿主RNA剪接进行重编程，以激活信号转导和转录激活因子3（STAT3）并驱动致癌性细胞转化。
mBio. 2025 Jun 12:e0129325. doi: 10.1128/mbio.01293-25.
7
Generation of chimeric forms of rhesus macaque rhadinovirus expressing KSHV envelope glycoproteins gH and gL for utilization in an NHP model of infection.构建表达卡波西肉瘤相关疱疹病毒包膜糖蛋白gH和gL的恒河猴疱疹病毒嵌合形式，用于非人灵长类动物感染模型。
J Virol. 2025 Feb 25;99(2):e0192324. doi: 10.1128/jvi.01923-24. Epub 2025 Jan 21.
8
Polypharmacology-based kinome screen identifies new regulators of KSHV reactivation.基于多药理学的激酶组筛选鉴定出卡波西肉瘤相关疱疹病毒重新激活的新调节因子。
bioRxiv. 2023 Feb 1:2023.02.01.526589. doi: 10.1101/2023.02.01.526589.
9
Essential function of the integrator complex in Kaposi's sarcoma-associated herpesvirus lytic replication.整合体复合物在卡波西肉瘤相关疱疹病毒裂解复制中的重要功能。
J Virol. 2025 Aug 13:e0026625. doi: 10.1128/jvi.00266-25.
10
KSHV and HPV modulate epithelial-to-mesenchymal transition in oral epithelial cells.卡波西肉瘤相关疱疹病毒和人乳头瘤病毒可调节口腔上皮细胞的上皮-间质转化。
mBio. 2025 Aug 15:e0048425. doi: 10.1128/mbio.00484-25.

本文引用的文献

1
Induced pluripotent stem cell-derived mesenchymal stem cells: whether they can become new stars of cell therapy.诱导多能干细胞衍生的间充质干细胞：它们能否成为细胞治疗的新明星。
Stem Cell Res Ther. 2024 Oct 16;15(1):367. doi: 10.1186/s13287-024-03968-x.
2
Kaposi's sarcoma-associated herpesvirus infection and its association with all-cause and cardiovascular mortality in the general adults: A prospective cohort study.卡波西肉瘤相关疱疹病毒感染及其与普通成年人全因和心血管死亡率的关系：一项前瞻性队列研究。
J Med Virol. 2024 Oct;96(10):e29953. doi: 10.1002/jmv.29953.
3
YAP in development and disease: Navigating the regulatory landscape from retina to brain.
YAP 在发育和疾病中的作用：从视网膜到大脑的调控景观探索。
Biomed Pharmacother. 2024 Jun;175:116703. doi: 10.1016/j.biopha.2024.116703. Epub 2024 May 6.
4
The evolving roles of Wnt signaling in stem cell proliferation and differentiation, the development of human diseases, and therapeutic opportunities.Wnt信号通路在干细胞增殖与分化、人类疾病发展及治疗机会方面不断演变的作用。
Genes Dis. 2023 Jul 22;11(3):101026. doi: 10.1016/j.gendis.2023.04.042. eCollection 2024 May.
5
A Kaposi's sarcoma-associated herpes virus-encoded microRNA contributes to dilated cardiomyopathy.卡波西肉瘤相关疱疹病毒编码的 microRNA 导致扩张型心肌病。
Signal Transduct Target Ther. 2023 Jun 9;8(1):226. doi: 10.1038/s41392-023-01434-3.
6
Neuropilin 1 is an entry receptor for KSHV infection of mesenchymal stem cell through TGFBR1/2-mediated macropinocytosis.神经纤毛蛋白 1 是 KSHV 感染间充质干细胞的进入受体，通过 TGFBR1/2 介导的巨胞饮作用。
Sci Adv. 2023 May 24;9(21):eadg1778. doi: 10.1126/sciadv.adg1778.
7
Crosstalk between the Hippo Pathway and the Wnt Pathway in Huntington's Disease and Other Neurodegenerative Disorders.Hippo 通路与 Wnt 通路在亨廷顿病和其他神经退行性疾病中的相互作用。
Cells. 2022 Nov 16;11(22):3631. doi: 10.3390/cells11223631.
8
Kaposi's sarcoma herpesvirus is associated with osteosarcoma in Xinjiang populations.卡波西肉瘤疱疹病毒与新疆人群中的骨肉瘤有关。
Proc Natl Acad Sci U S A. 2021 Mar 9;118(10). doi: 10.1073/pnas.2016653118.
9
Detection of Epstein-Barr Virus Infection in Non-Small Cell Lung Cancer.非小细胞肺癌中爱泼斯坦-巴尔病毒感染的检测
Cancers (Basel). 2019 May 31;11(6):759. doi: 10.3390/cancers11060759.
10
Evidence for Kaposi Sarcoma Originating from Mesenchymal Stem Cell through KSHV-induced Mesenchymal-to-Endothelial Transition.证据表明卡波西肉瘤源自间充质干细胞，通过 KSHV 诱导的间充质到内皮细胞的转变。
Cancer Res. 2018 Jan 1;78(1):230-245. doi: 10.1158/0008-5472.CAN-17-1961. Epub 2017 Oct 24.