Shimosegawa T, Kobari M, Yambe M, Koizumi M, Toyota T, Goto Y, Kobayashi S, Mochizuki T, Yanaihara C, Yanaihara N
Nihon Naibunpi Gakkai Zasshi. 1985 May 20;61(5):581-91. doi: 10.1507/endocrine1927.61.5_581.
The occurrence and localization of Met-enkephalin-Arg-Gly-Leu (Met-Enk-Arg-Gly-Leu)-like immunoreactivity in the lumbar paravertebral ganglion and the superior mesenteric ganglion in the rat were investigated by immunocytochemistry. Colchicine was employed as an axonal flow blocker to accumulate Met-Enk-Arg-Gly-Leu in the nerve cell bodies. The Met-Enk-Arg-Gly-Leu serum (R 0171) was produced by immunizing a mixed breed female rabbit with synthetic Met-Enk-Arg-Gly-Leu conjugated to ascaris protein by the glutaraldehyde method. Sections, 7 micron in thickness, of Bouin-fixed and paraffin-embedded tissues were immunostained by the peroxidase-antiperoxidase (PAP) method. Results obtained were summarized as follows: Colchicine-untreated rats possessed no strongly-immunoreactive nerve cell bodies for Met-Enk-Arg-Gly-Leu in the lumbar paravertebral ganglion, though a few of them were weakly immunopositive. Met-Enk-Arg-Gly-Leu-like immunoreactivity showing nerve fibers were occasionally seen in nerve fiber bundles of the lumbar paravertebral ganglion. In the superior mesenteric ganglion, a dense network of positively immunostained nerve fibers with a beaded appearance were seen around nerve cell bodies which were immunonegative for Met-Enk-Arg-Gly-Leu. In colchicine-treated rats, 55 percent of nerve cell bodies of the lumbar paravertebral ganglion showed various degrees of positive immunoreactivities for Met-Enk-Arg-Gly-Leu. Many strongly immunostained nerve terminals were seen in the lumbar paravertebral ganglion of colchicine-treated rats. In the superior mesenteric ganglion, the immunoreactivity for Met-Enk-Arg-Gly-Leu in the beaded nerve fibers around nerve cell bodies was intensified by the colchicine treatment, whereas the nerve cell bodies themselves remained immunonegative for Met-Enk-Arg-Gly-Leu.
采用免疫细胞化学方法研究了甲硫氨酸脑啡肽-精氨酸-甘氨酸-亮氨酸（Met-Enk-Arg-Gly-Leu）样免疫反应性在大鼠腰旁神经节和肠系膜上神经节中的发生及定位。秋水仙碱作为轴浆流阻断剂，用于使Met-Enk-Arg-Gly-Leu在神经细胞体中蓄积。Met-Enk-Arg-Gly-Leu血清（R 0171）是通过用戊二醛法将合成的Met-Enk-Arg-Gly-Leu与蛔虫蛋白偶联后免疫杂种雌性兔制备而成。将Bouin固定、石蜡包埋的组织切成7微米厚的切片，采用过氧化物酶-抗过氧化物酶（PAP）法进行免疫染色。结果总结如下：未用秋水仙碱处理的大鼠，腰旁神经节中没有对Met-Enk-Arg-Gly-Leu呈强免疫反应性的神经细胞体，不过有少数呈弱免疫阳性。在腰旁神经节的神经纤维束中偶尔可见显示神经纤维的Met-Enk-Arg-Gly-Leu样免疫反应性。在肠系膜上神经节中，在对Met-Enk-Arg-Gly-Leu呈免疫阴性的神经细胞体周围可见密集的、呈念珠状外观的阳性免疫染色神经纤维网络。在用秋水仙碱处理的大鼠中，腰旁神经节55%的神经细胞体对Met-Enk-Arg-Gly-Leu呈现不同程度的阳性免疫反应性。在用秋水仙碱处理的大鼠腰旁神经节中可见许多强免疫染色的神经终末。在肠系膜上神经节中，秋水仙碱处理增强了神经细胞体周围念珠状神经纤维中Met-Enk-Arg-Gly-Leu的免疫反应性，而神经细胞体本身对Met-Enk-Arg-Gly-Leu仍呈免疫阴性。
