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新鲜螺旋藻微胶囊对面条中蛋白质交联及结构变化影响的综合分析

Comprehensive analysis of the effects of fresh Spirulina microcapsules on protein cross-linking and structural changes in wheat noodles.

作者信息

Fan Zhihao, Shahid Ayesha, Su Kerui, Zhao Anqi, Zhang Beixiao, Xu Jingliang

机构信息

School of Chemical Engineering, Zhengzhou University, Zhengzhou 450001, China.

School of Chemical Engineering, Zhengzhou University, Zhengzhou 450001, China.; National Key Laboratory of Biobased Transportation Fuel Technology, Zhengzhou 450001, China.

出版信息

Food Chem. 2025 Aug 1;482:144034. doi: 10.1016/j.foodchem.2025.144034. Epub 2025 Mar 25.

DOI:10.1016/j.foodchem.2025.144034
PMID:40184750
Abstract

Fresh spirulina microcapsules (SP-MICs) are a viable strategy to prolong the preservation of fresh spirulina. This study aims to investigate the effects of SP-MICs on noodle quality, focusing on physicochemical properties and wheat gluten structures. Compared to the control group, the addition of 3 % SP-MICs significantly improved the sensory and textural properties of the noodles. Chewiness and hardness increased by 14.40 % and 32.36 %, respectively. In terms of glutenin subunits, HMS and B/C-LMS contents increased by 6.10 % and 5.39 %, respectively. Additionally, the gliadin-to-glutenin ratio reached its lowest at 1.19. Surface hydrophobicity decreased by 6.41 %, disulfide bond content increased by 31.60 %, and non-covalent interactions (hydrogen bond and hydrophobic interactions) were enhanced. The secondary structure of gluten protein became more ordered, with a 6.86 % increase in β-sheet content and a 1.10 % decrease in random coil content. SEM and CLSM results showed that 3 % SP-MICs significantly reinforced the gluten network and protein-starch cross-linking.

摘要

新鲜螺旋藻微胶囊(SP-MICs)是延长新鲜螺旋藻保存期的一种可行策略。本研究旨在探讨SP-MICs对面条品质的影响,重点关注其理化性质和小麦面筋结构。与对照组相比,添加3%的SP-MICs显著改善了面条的感官和质地特性。咀嚼性和硬度分别提高了14.40%和32.36%。就谷蛋白亚基而言,高分子量麦谷蛋白亚基(HMS)和B/C型低分子量麦谷蛋白亚基(B/C-LMS)含量分别增加了6.10%和5.39%。此外,醇溶蛋白与谷蛋白的比例最低达到1.19。表面疏水性降低了6.41%,二硫键含量增加了31.60%,非共价相互作用(氢键和疏水相互作用)增强。面筋蛋白的二级结构变得更加有序,β-折叠含量增加了6.86%,无规卷曲含量减少了1.10%。扫描电子显微镜(SEM)和共聚焦激光扫描显微镜(CLSM)结果表明,3%的SP-MICs显著增强了面筋网络和蛋白质-淀粉交联。

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