Microfluidic contact lens for continuous monitoring of ocular oxidative stress.

Oxidative stress-induced ocular dysfunctions are a significant global health concern. Glutathione (GSH), an abundant antioxidant in tears, can serve as an index for oxidative stress (OS). A coumarin-based fluorescent probe named CCAE was synthesized for GSH monitoring, functioning through structural changes via Michael's addition with GSH and elimination by HO, which restores the conjugation structure to assess the severity of OS-induced ocular diseases. CCAE demonstrated a high sensitivity with a detection limit (LOD) of 0.12 mM and reversibility for 15 cycles. A wearable contact lens sensor was developed featuring a microfluidic lens patterned with a 365 nm pulsed laser, requiring 6 μL of tears. CCAE was encapsulated in citric acid-crosslinked poly(vinyl alcohol) film and embedded in poly((hydroxyethyl)methacrylate-co-ethylene glycol)/polyvinylpyrrolidone (poly(HEMA-co-EG)/PVP) lenses. A customized smartphone readout device enabled quantitative GSH readings for point-of-care applications. Tested on an ex vivo porcine anterior eye model, the sensor achieved an LOD of 0.204 mM, within a detection range of 0.62-1.17 mM and 0.13-0.73 mM under mild and severe OS conditions respectively. The sensors maintained operational stability for 2 days and storage stability for 1 week. This reversible GSH contact lens sensor offers a unique platform for diagnosing and monitoring OS-related ocular conditions at point-of-care settings.