Guo Shaoke, Ding Ziqiang, Hu Liyan, Wang Xingdong, Cao Mengli, Kang Yandong, Zhang Ben, Pei Jie, Guo Xian
Key Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China; Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou 730050, China.
Key Laboratory of Yak Breeding in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China; Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lanzhou 730050, China.
Int J Biol Macromol. 2025 May;309(Pt 2):142813. doi: 10.1016/j.ijbiomac.2025.142813. Epub 2025 Apr 3.
Oocyte maturation requires close cooperation among multiple kinases and growth factors. However, the proteome landscape during yak oocyte maturation has not yet been characterized. Here, 4D-DIA (data independent acquisition) technology was used to sequence the proteome of yak oocytes at four stages, germinal vesicle (GV), germinal vesicle breakdown (GVBD), meiosis I (MI), and meiosis II (MII), and 5851 proteins were identified. During the transition from GV to GVBD, more proteins are activated to stimulate germinal vesicle breakdown. From MI to MII, the expression of most proteins was down-regulated. Bioinformatics analysis showed that GV oocytes contained a large number of proteins related to energy metabolism. The proteins in GVBD oocytes were mainly related to the activity of enzyme inhibitors. MII oocytes contained keratin family members and proteins involved in cell cycle regulation. In addition, it was found that the expression of phosphorylation modification enzymes and ubiquitination modification enzymes change dynamically during oocyte maturation. Parallel reaction monitoring (PRM) validated the expression of key differentially expressed proteins. These results provide important information for enhancing the understanding of the molecular mechanism of oocyte maturation and improving the efficiency of the in vitro maturation of yak oocytes, which are critically important for enhancing the reproductive efficiency of yak.
卵母细胞成熟需要多种激酶和生长因子之间密切协作。然而，牦牛卵母细胞成熟过程中的蛋白质组概况尚未得到描述。在此，利用4D-DIA（数据非依赖采集）技术对牦牛卵母细胞在生发泡（GV）、生发泡破裂（GVBD）、减数分裂I（MI）和减数分裂II（MII）四个阶段的蛋白质组进行测序，共鉴定出5851种蛋白质。在从GV向GVBD转变过程中，更多蛋白质被激活以刺激生发泡破裂。从MI到MII，大多数蛋白质的表达下调。生物信息学分析表明，GV期卵母细胞含有大量与能量代谢相关的蛋白质。GVBD期卵母细胞中的蛋白质主要与酶抑制剂活性相关。MII期卵母细胞含有角蛋白家族成员和参与细胞周期调控的蛋白质。此外，还发现磷酸化修饰酶和泛素化修饰酶的表达在卵母细胞成熟过程中动态变化。平行反应监测（PRM）验证了关键差异表达蛋白的表达。这些结果为增进对卵母细胞成熟分子机制的理解以及提高牦牛卵母细胞体外成熟效率提供了重要信息，这对提高牦牛繁殖效率至关重要。
