Tang Tingfan, Li Xuesong, Xu Ziwei, Zhao Jiayu, Zhan Yaqin, Li Yixing, Li Lijun, Cheng Hao, Tian Yuhong, Huang Wenyi, Feng Jun
Guangxi Key Laboratory of Green Processing of Sugar Resources, Department of Medicine/College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, Guangxi 545006, PR China; Provine and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry, Nanning, Guangxi 530004, PR China; Key Laboratory of Industrialized Processing and Safety of Guangxi Cuisine (Guilin Tourism University), Education Department of Guangxi Zhuang Autonomous Region, Guilin 541006, China; Guangxi Engineering Research Center for Large-Scale Preparation & Nutrients and Hygiene of Guangxi Cuisine, Guilin 541006, China.
Guangxi Key Laboratory of Green Processing of Sugar Resources, Department of Medicine/College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, Guangxi 545006, PR China.
J Chromatogr A. 2025 Jun 7;1750:465927. doi: 10.1016/j.chroma.2025.465927. Epub 2025 Apr 1.
In this study, magnetic multi-template molecularly imprinted polymers (MMIPs) are successfully synthesized. The synthesis is carried out by a multi-template surface imprinting strategy using mesoporous silica-coated magnetic graphene oxide as the support material, matrine (MT), oxymatrine (OMT) and sophocarpine (SC) as the templates, 4-vinylbenzoic acid as the functional monomer, and ethylene glycol dimethacrylate (EDGMA) as the cross-linker. The MMIPs were characterized using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The maximum adsorption capacity of the prepared MMIPs was found to be 9.01, 20.87, and 7.76 mg/g for MT, OMT, and SC, respectively, demonstrating higher adsorption capacity compared to the non-imprinted polymers (MNIPs). Furthermore, MMIPs were utilized as adsorbents for magnetic solid-phase extraction (MSPE) in conjunction with pressurized capillary electrochromatography (pCEC) for the simultaneous separation and detection of MT, OMT, and SC in the Chinese herbal medicine substrate sophora. The factors affecting the extraction efficiency of MSPE, including the type of elution solvent, amount of adsorbent, pH of the sample solution, elution time, and eluent volume, were optimized. The MMIPs-pCEC method demonstrated a satisfactory linear response within the concentration range of 1-50 µg/mL for the three alkaloids, with correlation coefficients ranging from 0.9923 to 0.9972 and limits of detection ranging from 0.09 to 0.73 µg/mL. The findings of the present study demonstrate that MMIPs have important applications in the analysis of active ingredients in traditional Chinese medicine.
在本研究中，成功合成了磁性多模板分子印迹聚合物（MMIPs）。合成过程采用多模板表面印迹策略，以介孔二氧化硅包覆的磁性氧化石墨烯为载体材料，苦参碱（MT）、氧化苦参碱（OMT）和槐果碱（SC）为模板，4-乙烯基苯甲酸为功能单体，乙二醇二甲基丙烯酸酯（EDGMA）为交联剂。使用傅里叶变换红外光谱、X射线衍射、扫描电子显微镜和透射电子显微镜对MMIPs进行了表征。发现制备的MMIPs对MT、OMT和SC的最大吸附容量分别为9.01、20.87和7.76 mg/g，表明其吸附容量高于非印迹聚合物（MNIPs）。此外，MMIPs被用作磁性固相萃取（MSPE）的吸附剂，并与加压毛细管电色谱（pCEC）结合，用于同时分离和检测中药基质苦参中的MT、OMT和SC。对影响MSPE萃取效率的因素进行了优化，包括洗脱溶剂类型、吸附剂用量、样品溶液pH值、洗脱时间和洗脱液体积。MMIPs-pCEC方法在三种生物碱1-50 µg/mL的浓度范围内表现出令人满意的线性响应，相关系数在0.9923至0.9972之间，检测限在0.09至0.73 µg/mL之间。本研究结果表明，MMIPs在中药活性成分分析中具有重要应用。
