在甘油和TESTCY中冷冻保存的人类精子的超微结构分类

Ultrastructural categorization of human sperm cryopreserved in glycerol and in TESTCY.

作者信息

Heath E, Jeyendran R S, Perez-Pelaez M, Sobrero A J

出版信息

Int J Androl. 1985 Apr;8(2):101-10. doi: 10.1111/j.1365-2605.1985.tb00823.x.

DOI:10.1111/j.1365-2605.1985.tb00823.x

Abstract

The motility of human sperm after thawing and the ultrastructural integrity of their acrosomal region was evaluated in 5 semen donors after utilizing 2 cyproprotective extenders--glycerol and TESTCY. Sperm motility in the fresh control semen fraction (65.5% +/- 6.1) was significantly (P less than 0.05) better than the immediate post-thaw motility in both cyprotected fractions (38.8% +/- 2.0 for glycerol and 31.0% +/- 2.9 for TESTCY), and there was no significant difference between the two cyprotective treatments. The overall % of normal intact sperm heads was significantly less than in controls (P less than 0.05) following cypropreservation in glycerol, but values for sperm stored in TESTCY were not significantly different from controls or glycerol. However, when expressed as % change from controls values, sperm preservation was significantly better (P less than 0.05) in TESTCY than in glycerol. TESTCY is considered a better cryoprotective agent for human sperm.

摘要

在5名精液捐献者中，使用两种冷冻保护剂——甘油和TESTCY后，对解冻后人类精子的活力及其顶体区域的超微结构完整性进行了评估。新鲜对照精液样本中的精子活力（65.5%±6.1）显著（P<0.05）高于两种冷冻保护剂处理后的解冻后即刻活力（甘油处理组为38.8%±2.0，TESTCY处理组为31.0%±2.9），且两种冷冻保护处理之间无显著差异。甘油冷冻保存后，正常完整精子头部的总体百分比显著低于对照组（P<0.05），但TESTCY保存的精子的值与对照组或甘油组无显著差异。然而，以相对于对照值的百分比变化表示时，TESTCY中精子的保存情况显著优于甘油组（P<0.05）。TESTCY被认为是人类精子更好的冷冻保护剂。