Han Jing, Huang Congfu, Meng Lingjuan, Wu Hui, Meng Dongming
Department of Pediatrics, Longgang Maternity and Child Institute of Shantou University Medical College (Longgang District Maternity & Child Healthcare Hospital of Shenzhen City), Shenzhen 518172, China.
Department of Pediatrics, Hexian Memorial Affiliated Hospital of Southern Medical University, Guangzhou 511400, China.
Endocr J. 2025 Jul 1;72(7):791-799. doi: 10.1507/endocrj.EJ24-0615. Epub 2025 Apr 5.
Children with idiopathic short stature (ISS) and growth hormone deficiency (GHD) exhibit imbalances in gut microbiota (GM), and the latter is related to endocrine hormones (such as insulin-like growth factor 1 (IGF-1)). The current study investigated the compositional and functional variations in GM between children with ISS and GHD, employing 16S rRNA sequencing technology. Sequencing results from 15 children with ISS and 18 children with GHD indicated no significant differences in GM alpha diversity or phylum-level diversity between the ISS and GHD groups. At the genus level, the abundance of Terrisporobacter was significantly greater in the ISS group compared to the GHD group, whereas the abundance of Acidovorax was reduced. The abundance of Prevotella stercorea and uncultured Sutterella sp. at the species level was significantly lower in the ISS group compared to the GHD group. The third level (L3) of the Kyoto Encyclopedia of Genes and Genomes (KEGG) database revealed functional variations in GM, with children in the ISS group having higher levels of intestinal bacteria Mobility Proteins and Background Chemotaxis. Despite these differences, the overall composition and function of GM between ISS and GHD children were not significantly different, indicating that the mechanisms by which GM influences the growth and development of children in both groups may be similar. This study was registered with the Medical Research Registration and Record System with the registration number MR-44-24-045472.
患有特发性身材矮小（ISS）和生长激素缺乏症（GHD）的儿童肠道微生物群（GM）存在失衡，且后者与内分泌激素（如胰岛素样生长因子1（IGF-1））有关。本研究采用16S rRNA测序技术，调查了ISS和GHD儿童GM的组成和功能变化。15名ISS儿童和18名GHD儿童的测序结果表明，ISS组和GHD组在GM的α多样性或门水平多样性方面无显著差异。在属水平上，与GHD组相比，ISS组中Terrisporobacter的丰度显著更高，而Acidovorax的丰度降低。与GHD组相比，ISS组中在种水平上的粪便普雷沃氏菌和未培养的萨特氏菌属丰度显著更低。京都基因与基因组百科全书（KEGG）数据库的第三层（L3）显示了GM的功能变化，ISS组儿童的肠道细菌移动蛋白和背景趋化性水平更高。尽管存在这些差异，但ISS和GHD儿童GM的总体组成和功能并无显著差异，这表明GM影响两组儿童生长发育的机制可能相似。本研究已在医学研究注册与记录系统注册，注册号为MR-44-24-045472。
