Floridi A, Palmerini C A, Fini C, Pupita M, Fidanza F
Int J Vitam Nutr Res. 1985;55(2):187-91.
A method for the determination of Flavin Adenine Dinucleotide in whole blood is presented. The method comprises extraction of the vitamer by using a 6% trichloroacetic acid-20% acetonitrile mixture and direct analysis of the sample extract by using a straight-phase high performance liquid chromatography. A linear calibration curve was obtained for standard vitamer and a satisfactory recovery was observed from spiked samples. Evidence for reproducibility and precision of the assay is presented. The method was applied to determine vitamer levels in blood of healthy subjects.
本文介绍了一种测定全血中黄素腺嘌呤二核苷酸的方法。该方法包括用6%三氯乙酸-20%乙腈混合物提取维生素原，并使用正相高效液相色谱法直接分析样品提取物。得到了标准维生素原的线性校准曲线，加标样品的回收率良好。给出了该测定方法的重现性和精密度证据。该方法用于测定健康受试者血液中的维生素原水平。
