Mori H, Konishi T
Hear Res. 1985 Mar;17(3):227-36. doi: 10.1016/0378-5955(85)90067-x.
The endocochlear potential and Cl- activities in the endolymph and perilymph of guinea pigs were simultaneously measured with a pair of double-barreled Cl--sensitive liquid-membrane electrodes. Under normal conditions the mean Cl- activity was 96.6 mEg/l in the endolymph and 93.5 mEq/l in the perilymph. The mean Cl- concentration determined by potentiometric titration was 132.3 mM in the endolymph and 121.8 mM in the perilymph. The permeability to Cl- of the cochlear partition was determined after active transport was abolished by permanent anoxia. The modified permeability coefficient and conductance for Cl- of the cochlear partition were computed from the rate of decrease in the Cl- concentration of the endolymph and its electrochemical potential difference between the endolymph and perilymph. The mean modified Cl- conductance and permeability coefficient of the cochlear partition were (10.7 +/- 4.25) X 10(-3) omega -1 . cm-3 and (22.58 +/- 8.95) X 10(-6) s-1 respectively, when averaged from 10 to 30 min after onset of anoxia. Our results indicate that the cochlear partition is relatively permeable to Cl-, when compared to its permeability to K+. Possible coupling between Cl- and water movement across the cochlear partition is discussed.
用一对双管氯离子敏感液膜电极同时测量豚鼠内淋巴和外淋巴中的内耳蜗电位及氯离子活性。在正常情况下，内淋巴中氯离子活性的平均值为96.6毫当量/升，外淋巴中为93.5毫当量/升。通过电位滴定法测定的内淋巴中氯离子浓度平均值为132.3毫摩尔/升，外淋巴中为121.8毫摩尔/升。在永久性缺氧消除主动转运后，测定了耳蜗隔板对氯离子的通透性。根据内淋巴中氯离子浓度的降低速率及其在内淋巴和外淋巴之间的电化学势差，计算出耳蜗隔板对氯离子的修正通透性系数和电导率。缺氧开始后10至30分钟平均计算，耳蜗隔板的平均修正氯离子电导率和通透性系数分别为(10.7±4.25)×10(-3)Ω-1.cm-3和(22.58±8.95)×10(-6)s-1。我们的结果表明，与耳蜗隔板对钾离子的通透性相比，其对氯离子的通透性相对较高。文中还讨论了氯离子和水跨耳蜗隔板移动之间可能的耦合关系。
