Muzeni R J
J Assoc Off Anal Chem. 1985 May-Jun;68(3):506-8.
Isothermal gas chromatography with flame ionization detection was used to determine residual ethylene oxide (EtO), ethylene chlorohydrin, and ethylene glycol in soft rubber catheters that had been sterilized with EtO. Catheter samples were extracted by shaking with carbon disulfide, and the extract was analyzed on a 3% Carbowax 20M on 80-100 mesh Chromosorb 101 column, using nitrogen as the carrier gas. Ten replicate injections of a mixed standards solution gave coefficients of variation of 1.91, 1.23, and 4.74% for EtO, ethylene chlorohydrin, and ethylene glycol, respectively. A linear response was obtained with concentrations ranging from 1.0 to 7.9 micrograms EtO, 14.0 to 88.0 micrograms ethylene chlorohydrin, and 31.0 to 98.5 micrograms ethylene glycol. The proposed method detected as little as 0.5, 5.0, and 16.5 ng EtO, ethylene chlorohydrin, and ethylene glycol, respectively.
采用带有火焰离子化检测的等温气相色谱法来测定经环氧乙烷（EtO）灭菌的软质橡胶导管中的环氧乙烷残留量、氯乙醇和乙二醇。导管样品通过与二硫化碳振荡萃取，萃取液在涂有3%聚乙二醇20M的80 - 100目Chromosorb 101柱上进行分析，以氮气作为载气。对混合标准溶液进行十次重复进样，环氧乙烷、氯乙醇和乙二醇的变异系数分别为1.91%、1.23%和4.74%。在环氧乙烷浓度范围为1.0至7.9微克、氯乙醇浓度范围为14.0至88.0微克、乙二醇浓度范围为31.0至98.5微克时获得线性响应。所提出的方法分别能检测低至0.5纳克、5.0纳克和16.5纳克的环氧乙烷、氯乙醇和乙二醇。
