Cieri U R
J Assoc Off Anal Chem. 1985 May-Jun;68(3):542-4.
A procedure is presented for the determination of reserpine in commercial tablets by liquid chromatography (LC). The sample is extracted with methanol if only reserpine is present. If the sample contains other ingredients, CHCI3 is used for extraction from aqueous suspension; the CHCI3 is subsequently completely evaporated in the presence of methanol. For LC, a normal phase column, methanol as the eluting solvent, and a fluorometric detector are used. A recovery study indicated that no measurable degradation of reserpine occurs during evaporation of the CHCI3 extract. Several commercial tablets containing reserpine alone or in combination with other ingredients were analyzed by the proposed method, and the results were compared with those obtained by the current official USP methods for reserpine.
介绍了一种通过液相色谱法（LC）测定市售片剂中利血平的方法。如果仅存在利血平，则用甲醇提取样品。如果样品含有其他成分，则用CHCI3从水悬浮液中提取；随后在甲醇存在下将CHCI3完全蒸发。对于LC，使用正相柱、甲醇作为洗脱溶剂和荧光检测器。回收率研究表明，在CHCI3提取物蒸发过程中，利血平没有发生可测量的降解。用所提出的方法分析了几种单独含有利血平或与其他成分组合的市售片剂，并将结果与目前美国药典中利血平的官方方法所得结果进行了比较。
