Akerblom L, Reichard P
J Biol Chem. 1985 Aug 5;260(16):9197-202.
2'-Azidocytidine is a specific inhibitor of DNA synthesis in cultured 3T6 mouse fibroblasts. Earlier work (Akerblom, L., Pontis, E., and Reichard, P. (1982) 257, 6776-6782) indicated that the nucleoside, after phosphorylation, acted by inhibiting both ribonucleotide reduction and DNA strand elongation. We now demonstrate that the effect on strand elongation was due to a contamination of azidocytidine with less than 0.3% of arabinosyl cytosine. Pure azidocytidine inhibits specifically ribonucleotide reductase and its effects on DNA synthesis are secondary to this inhibition. The results with azidocytidine concerning the size and turnover of deoxyribonucleoside triphosphate pools parallel those of hydroxyurea and are distinct from those of arabinosyl cytosine. Together with hydroxyurea, azidocytidine is a useful compound in studies aiming at a specific block of the production of deoxynucleoside triphosphates in intact cells. Comparisons of the effects of azidocytidine and arabinosyl cytosine complement earlier studies with hydroxyurea and aphidicolin concerning inter-relations between dNTP synthesis and DNA replication.
2'-叠氮胞苷是培养的3T6小鼠成纤维细胞中DNA合成的特异性抑制剂。早期研究（阿克布洛姆，L.，庞蒂斯，E.，和赖夏德，P.（1982年）257，6776 - 6782）表明，该核苷磷酸化后通过抑制核糖核苷酸还原和DNA链延伸发挥作用。我们现在证明，对链延伸的影响是由于叠氮胞苷被低于0.3%的阿糖胞苷污染所致。纯叠氮胞苷特异性抑制核糖核苷酸还原酶，其对DNA合成的影响是这种抑制的继发效应。关于脱氧核苷三磷酸池的大小和周转，叠氮胞苷的结果与羟基脲的结果相似，与阿糖胞苷的结果不同。与羟基脲一起，叠氮胞苷是旨在特异性阻断完整细胞中脱氧核苷三磷酸产生的研究中的一种有用化合物。叠氮胞苷和阿糖胞苷作用的比较补充了早期关于羟基脲和阿非科林对dNTP合成与DNA复制之间相互关系的研究。
