Schor S L, Schor A M, Rushton G, Smith L
J Cell Sci. 1985 Feb;73:221-34. doi: 10.1242/jcs.73.1.221.
Data are presented indicating that the migration of fibroblasts into three-dimensional collagen gels is affected by cell density. We have defined a 'cell density migration index' (CDMI) to express this behavioural response in quantitative terms. The results of a survey of 77 different cell types indicate that the CDMI values expressed by normal adult skin fibroblasts and transformed cell lines fall into two distinct, non-overlapping groups. Measurement of the CDMI therefore provides an additional means of distinguishing between normal and transformed cells and may be used in conjunction with other commonly recognized criteria (e.g. anchorage-independent growth) to assess expression of a transformed phenotype in vitro. It is of interest to note that the CDMI values expressed by foetal cells define a group lying intermediate between normal and transformed cells. Both uncloned and cloned foetal cells have been observed to undergo a stable transition to expression of CDMI values characteristic of adult cells when followed throughout the duration of their in vitro lifespan. In addition to providing a novel means of distinguishing between normal adult and foetal cells, our results suggest that foetal fibroblasts undergo an 'isoformic' transition at some point in their developmental history, which is manifest in vitro by the expression of an adult CDMI.
所呈现的数据表明，成纤维细胞向三维胶原凝胶中的迁移受细胞密度影响。我们定义了一个“细胞密度迁移指数”（CDMI）来定量表达这种行为反应。对77种不同细胞类型的调查结果表明，正常成年皮肤成纤维细胞和转化细胞系所表达的CDMI值分为两个不同的、不重叠的组。因此，CDMI的测量提供了一种区分正常细胞和转化细胞的额外方法，并且可与其他普遍认可的标准（如不依赖贴壁生长）结合使用，以评估体外转化表型的表达。值得注意的是，胎儿细胞所表达的CDMI值定义了一个介于正常细胞和转化细胞之间的组。在整个体外寿命期间跟踪观察到，未克隆和克隆的胎儿细胞都会稳定地转变为表达成年细胞特征性的CDMI值。除了提供一种区分正常成年细胞和胎儿细胞的新方法外，我们的结果表明，胎儿成纤维细胞在其发育历史的某个阶段经历了“同种型”转变，这在体外表现为成年CDMI的表达。
