Kim Hyunsoo, Takegahara Noriko, Choi Yongwon
Department of Pathology and Laboratory Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
MicroPubl Biol. 2025 Mar 24;2025. doi: 10.17912/micropub.biology.001469. eCollection 2025.
Osteoclasts are primary bone resorbing cells. Previously, we described metabolic regulation of osteoclasts through IgSF11-mediated phosphorylation of the glycolytic enzyme PKM2. Here, we report the impact of IgSF11-PKM2-mediated regulation on gene expression in osteoclasts, utilizing RNA sequencing on osteoclasts engineered to express a chimeric protein, lacking IgSF11, and pharmacologically modulating PKM2 activity. Our analysis identified osteoclast-related genes whose expression is altered by the absence of IgSF11 and by changes in PKM2 activity. This study reveals gene expression changes associated with the IgSF11-PKM2 pathway, providing new insight into its role in osteoclasts.
破骨细胞是主要的骨吸收细胞。此前，我们描述了通过免疫球蛋白超家族成员11（IgSF11）介导的糖酵解酶丙酮酸激酶M2（PKM2）磷酸化对破骨细胞的代谢调节。在此，我们利用对经基因工程改造以表达缺乏IgSF11的嵌合蛋白的破骨细胞进行RNA测序，并通过药理学方法调节PKM2活性，报告了IgSF11 - PKM2介导的调节对破骨细胞基因表达的影响。我们的分析确定了与破骨细胞相关的基因，其表达因IgSF11的缺失和PKM2活性的变化而改变。这项研究揭示了与IgSF11 - PKM2途径相关的基因表达变化，为其在破骨细胞中的作用提供了新的见解。
