Inoue T, Niwaguchi T
J Chromatogr. 1985 Apr 12;339(1):163-9. doi: 10.1016/s0378-4347(00)84638-1.
A method for the direct quantitative densitometry of nitrazepam and its main metabolites (7-aminonitrazepam, 7-acetamidonitrazepam and 2-amino-5-nitrobenzophenone) in urine was developed. The unchanged drug and its metabolites were extracted with benzene-dichloromethane (4:1), subjected to thin-layer chromatography, and determined by direct ultraviolet densitometry. Recovery experiments showed that the method was quantitative. The limit of detection was 5 ng/ml for 2-amino-5-nitrobenzophenone and 10 ng/ml for other compounds. The method was applied to the determination of nitrazepam and its metabolites excreted in human urine after administration of 10 mg of the drug.
建立了一种直接定量测定尿液中硝西泮及其主要代谢物（7-氨基硝西泮、7-乙酰氨基硝西泮和2-氨基-5-硝基二苯甲酮）的密度测定法。未变化的药物及其代谢物用苯 - 二氯甲烷（4:1）萃取，进行薄层色谱分析，并通过直接紫外密度测定法测定。回收率实验表明该方法是定量的。2-氨基-5-硝基二苯甲酮的检测限为5 ng/ml，其他化合物为10 ng/ml。该方法用于测定给药10 mg药物后人体尿液中排出的硝西泮及其代谢物。
