Wang Junyue, Liu Qian, Huang Shuning, Mertens Kenneth Neil, Pospelova Vera, Shen Xin, Gu Haifeng
School of Marine Sciences, Nanjing University of Information Science and Technology, Nanjing, 210044, China; Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China.
School of Marine Sciences, Nanjing University of Information Science and Technology, Nanjing, 210044, China.
Mar Pollut Bull. 2025 Jun;215:117899. doi: 10.1016/j.marpolbul.2025.117899. Epub 2025 Apr 8.
Resting cysts of dinoflagellates can persist in sediments, seeding harmful algal blooms (HABs). A DNA metabarcoding approach was employed, targeting the large subunit ribosomal (LSU D1-D2) and the internal transcribed spacer (ITS1) to investigate the diversity and biogeography of dinoflagellate cysts from the South China Sea to the Chukchi Sea. The LSU and ITS1 datasets identified 196 and 118 species, respectively, with only 59 dinoflagellate cyst species revealed by both approaches. Eleven cyst species of potentially toxic dinoflagellates and 82 species previously unknown as cyst producers were detected. Cysts of Heterocapsa cf. horiguchii, Heterocapsa minima, Heterocapsa iwatakii, Heterocapsa rotundata, and Heterocapsa steinii were documented through germination for the first time, with the latter three species also detected via metabarcoding. This study provides critical insights into the diversity and biogeography of dinoflagellate cysts by highlighting the complementary detection capabilities of LSU and ITS1 molecular markers and their trans-latitudinal distribution patterns. The identification of potentially toxic cysts and their ecological distributions offers crucial information on the ecology of harmful dinoflagellates. These findings underscore the importance of molecular techniques in monitoring dinoflagellate cysts.
甲藻的休眠孢囊可以在沉积物中持续存在，引发有害藻华（HABs）。本研究采用DNA宏条形码方法，靶向大亚基核糖体（LSU D1-D2）和内部转录间隔区（ITS1），以研究从南海到楚科奇海的甲藻孢囊的多样性和生物地理学。LSU和ITS1数据集分别鉴定出196种和118种，两种方法仅揭示了59种甲藻孢囊物种。检测到11种潜在有毒甲藻的孢囊物种和82种以前未知的孢囊产生物种。首次通过萌发记录了霍氏异帽藻、微小异帽藻、岩手异帽藻、圆形异帽藻和施泰尼异帽藻的孢囊，后三种物种也通过宏条形码检测到。本研究通过强调LSU和ITS1分子标记的互补检测能力及其跨纬度分布模式，为甲藻孢囊的多样性和生物地理学提供了关键见解。潜在有毒孢囊的鉴定及其生态分布提供了关于有害甲藻生态学的关键信息。这些发现强调了分子技术在监测甲藻孢囊方面的重要性。
