• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

整合多组学技术鉴定海鞘脊索管腔形成的关键信号通路

Integrated multi-omics identify key signalling pathways for notochord lumenogenesis in ascidian .

作者信息

Zhang Jin, Tan Zicheng, Qin Qishu, Peng Hongzhe, Shi Wenjie, Yu Haiyan, Dong Bo

机构信息

Fang Zongxi Center for Marine EvoDevo, MoE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, People's Republic of China.

Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, People's Republic of China.

出版信息

Open Biol. 2025 Apr;15(4):240402. doi: 10.1098/rsob.240402. Epub 2025 Apr 9.

DOI:10.1098/rsob.240402
PMID:40199341
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11978462/
Abstract

Lumen formation and inflation are crucial for tubular organ morphogenesis and function. However, the key signalling pathways for lumenogenesis regulation were not well identified. Here, we performed tissue-specific transcriptomic sequencing for the isolated notochord tissue, in which 10 551 genes in total were identified. To investigate crucial signalling pathways in regulating lumenogenesis, KEGG was performed and the results showed that the Rap1 signalling pathway, vascular endothelial growth factor signalling pathway, mitogen activated protein kinase (MAPK) signalling pathway (plant) and Ras signalling pathway might play important roles in lumenogenesis. Moreover, correlation analysis with proteomic data and comparison analysis of single-cell transcriptomic data were further utilized to identify the potential critical roles of the Rap1 signalling pathway and Ras signalling pathway in lumenogenesis. To verify their functions in lumenogenesis, the Ras/calcium-Rap1-MAPK signalling axis was blocked, and the results showed that the notochord lumenogenesis failed. Meanwhile, we identified that CDC42 was a potential downstream target factor of the Ras-Rap1-MAPK signalling axis, playing crucial functions in notochord lumenogenesis. Overall, we systematically revealed the key regulatory signalling pathways for notochord lumen formation and verified a lumenogenesis-related signalling axis, providing a foundational data resource for exploring the mechanisms of lumenogenesis.

摘要

管腔形成和扩张对于管状器官的形态发生和功能至关重要。然而，管腔发生调控的关键信号通路尚未得到很好的确定。在此，我们对分离的脊索组织进行了组织特异性转录组测序，共鉴定出10551个基因。为了研究调控管腔发生的关键信号通路，我们进行了KEGG分析，结果表明Rap1信号通路、血管内皮生长因子信号通路、丝裂原活化蛋白激酶（MAPK）信号通路（植物）和Ras信号通路可能在管腔发生中发挥重要作用。此外，我们进一步利用蛋白质组学数据的相关性分析和单细胞转录组数据的比较分析，来确定Rap1信号通路和Ras信号通路在管腔发生中的潜在关键作用。为了验证它们在管腔发生中的功能，我们阻断了Ras/钙-Rap1-MAPK信号轴，结果表明脊索管腔发生失败。同时，我们确定CDC42是Ras-Rap1-MAPK信号轴的一个潜在下游靶因子，在脊索管腔发生中发挥关键作用。总体而言，我们系统地揭示了脊索管腔形成的关键调控信号通路，并验证了一个与管腔发生相关的信号轴，为探索管腔发生机制提供了基础数据资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/b632b0283857/rsob.240402.f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/ff1d6112cb12/rsob.240402.fg002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/dece8c2e6811/rsob.240402.f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/ff6bde8f8d5e/rsob.240402.f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/cb88a0702f9d/rsob.240402.f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/391c71fb6e59/rsob.240402.f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/c0a0f48806b3/rsob.240402.f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/b632b0283857/rsob.240402.f006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/ff1d6112cb12/rsob.240402.fg002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/dece8c2e6811/rsob.240402.f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/ff6bde8f8d5e/rsob.240402.f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/cb88a0702f9d/rsob.240402.f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/391c71fb6e59/rsob.240402.f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/c0a0f48806b3/rsob.240402.f005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b11/11978462/b632b0283857/rsob.240402.f006.jpg

相似文献

1
Integrated multi-omics identify key signalling pathways for notochord lumenogenesis in ascidian .整合多组学技术鉴定海鞘脊索管腔形成的关键信号通路
Open Biol. 2025 Apr;15(4):240402. doi: 10.1098/rsob.240402. Epub 2025 Apr 9.
2
Proteomic identification of intracellular vesicle trafficking and protein glycosylation requirements for lumen inflation in Ciona notochord.文昌鱼脊索内囊泡运输和蛋白糖基化的蛋白质组学鉴定。
Proteomics. 2023 May;23(10):e2200460. doi: 10.1002/pmic.202200460. Epub 2023 Feb 25.
3
MiR-92 Family Members Form a Cluster Required for Notochord Tubulogenesis in Urochordate .miR-92 家族成员构成了环节动物体节中脊索管状形成所必需的簇。
Genes (Basel). 2021 Mar 12;12(3):406. doi: 10.3390/genes12030406.
4
Quantitative Phosphoproteomics Reveals the Requirement of DYRK1-Mediated Phosphorylation of Ion Transport- and Cell Junction-Related Proteins for Notochord Lumenogenesis in Ascidian.定量磷酸化蛋白质组学揭示了 DYRK1 介导的离子转运和细胞连接相关蛋白磷酸化对文昌鱼脊索管腔发生的需求。
Cells. 2023 Mar 16;12(6):921. doi: 10.3390/cells12060921.
5
Nuclear Factor of Activated T Cells-5 Regulates Notochord Lumenogenesis in Chordate Larval Development.激活 T 细胞核因子-5 调控脊索动物幼虫发育中的脊索管腔生成。
Int J Mol Sci. 2022 Nov 19;23(22):14407. doi: 10.3390/ijms232214407.
6
The notochord gene regulatory network in chordate evolution: Conservation and divergence from Ciona to vertebrates.脊索动物进化中的脊索基因调控网络：从海鞘到脊椎动物的保守性和分歧性。
Curr Top Dev Biol. 2020;139:325-374. doi: 10.1016/bs.ctdb.2020.01.002. Epub 2020 Feb 12.
7
The Ciona Notochord Gene Regulatory Network.海鞘脊索基因调控网络。
Results Probl Cell Differ. 2018;65:163-184. doi: 10.1007/978-3-319-92486-1_9.
8
Fine-Tuned Expression of Evolutionarily Conserved Signaling Molecules in the Notochord.脊索中进化保守信号分子的微调表达
Int J Mol Sci. 2024 Dec 20;25(24):13631. doi: 10.3390/ijms252413631.
9
Dynamics of Chromatin Opening across Larval Development in the Urochordate Ascidian .原肠胚发育期文昌鱼染色质开放性的动态变化
Int J Mol Sci. 2024 Feb 28;25(5):2793. doi: 10.3390/ijms25052793.
10
Functional and evolutionary insights from the notochord transcriptome.来自脊索转录组的功能与进化见解
Development. 2017 Sep 15;144(18):3375-3387. doi: 10.1242/dev.156174.

本文引用的文献

1
OmicShare tools: A zero-code interactive online platform for biological data analysis and visualization.奥米共享工具：一个用于生物数据分析和可视化的零代码交互式在线平台。
Imeta. 2024 Aug 1;3(5):e228. doi: 10.1002/imt2.228. eCollection 2024 Oct.
2
Targeting the RAS/RAF/MAPK pathway for cancer therapy: from mechanism to clinical studies.靶向 RAS/RAF/MAPK 通路治疗癌症：从机制到临床研究。
Signal Transduct Target Ther. 2023 Dec 18;8(1):455. doi: 10.1038/s41392-023-01705-z.
3
Establishment of a developmental atlas and transgenetic tools in the ascidian .
在海鞘中建立发育图谱和转基因工具。
Mar Life Sci Technol. 2023 Nov 22;5(4):435-454. doi: 10.1007/s42995-023-00200-2. eCollection 2023 Nov.
4
A collagen-rich arch in the urochordate notochord coordinates cell shaping and multi-tissue elongation.脊索动物富含胶原蛋白的脊索拱协调细胞塑形和多组织伸长。
Curr Biol. 2023 Dec 18;33(24):5390-5403.e3. doi: 10.1016/j.cub.2023.11.001. Epub 2023 Nov 22.
5
An Ascidian Homolog of the Mouse Brachyury (T) Gene is Expressed Exclusively in Notochord Cells at the Fate Restricted Stage: (Ascidians/T (Brachyury) gene/sequence conservation/notochord cells/transient expression).小鼠短尾（T）基因的海鞘同源物在命运受限阶段仅在脊索细胞中表达：（海鞘/T（短尾）基因/序列保守性/脊索细胞/瞬时表达）
Dev Growth Differ. 1994 Feb;36(1):9-18. doi: 10.1111/j.1440-169X.1994.00009.x.
6
SHP2 Inhibition Sensitizes Diverse Oncogene-Addicted Solid Tumors to Re-treatment with Targeted Therapy.SHP2 抑制增强了多种致癌基因依赖的实体肿瘤对靶向治疗的再治疗敏感性。
Cancer Discov. 2023 Aug 4;13(8):1789-1801. doi: 10.1158/2159-8290.CD-23-0361.
7
Proteomic identification of intracellular vesicle trafficking and protein glycosylation requirements for lumen inflation in Ciona notochord.文昌鱼脊索内囊泡运输和蛋白糖基化的蛋白质组学鉴定。
Proteomics. 2023 May;23(10):e2200460. doi: 10.1002/pmic.202200460. Epub 2023 Feb 25.
8
EDomics: a comprehensive and comparative multi-omics database for animal evo-devo.EDomics：一个用于动物进化发育的全面和比较多组学数据库。
Nucleic Acids Res. 2023 Jan 6;51(D1):D913-D923. doi: 10.1093/nar/gkac944.
9
FGFR1/MAPK-directed brachyury activation drives PD-L1-mediated immune evasion to promote lung cancer progression.FGFR1/MAPK 靶向的 brachyury 激活驱动 PD-L1 介导的免疫逃逸，促进肺癌进展。
Cancer Lett. 2022 Oct 28;547:215867. doi: 10.1016/j.canlet.2022.215867. Epub 2022 Aug 17.
10
Hiplot: a comprehensive and easy-to-use web service for boosting publication-ready biomedical data visualization.Hiplot：一个全面且易于使用的网络服务，用于增强可发表的生物医学数据可视化效果。
Brief Bioinform. 2022 Jul 18;23(4). doi: 10.1093/bib/bbac261.