Zhao Xinfang, Fang Huan, Dong Ning, Kong Kaize, Zhang Jijiao, Fan Xiaoguang, Zhang Dawei
College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
J Agric Food Chem. 2025 Apr 23;73(16):9732-9742. doi: 10.1021/acs.jafc.5c00078. Epub 2025 Apr 8.
Vitamin B has garnered increasing interest in biotechnological applications due to its critical role in human health and economic benefits. In this study, heterologous vitamin B biosynthetic pathway genes were integrated into the chromosome of . Cofactor engineering significantly enhanced production levels by expressing the hemoglobin gene from for improved oxygen transfer and substituting the Embden-Meyerhof-Parnas pathway with the Entner-Doudoroff pathway to increase ATP and NADPH availability. The optimal strain produced 3.54 mg/L of vitamin B in a respiration activity monitoring system. Further optimization of fermentation media through single-factor and Taguchi methods resulted in a significant increase in production, achieving a remarkable 21.09 mg/L in a 5 L fermenter containing a low-cost defined medium. These findings demonstrate the potential of engineered strains for industrial vitamin B production, offering a cost-effective and efficient biotechnological solution.
维生素B因其在人类健康中的关键作用和经济效益，在生物技术应用中受到越来越多的关注。在本研究中，异源维生素B生物合成途径基因被整合到……的染色体中。通过表达来自……的血红蛋白基因以改善氧气传递，并将糖酵解途径替换为Entner-Doudoroff途径以增加ATP和NADPH的可用性，辅因子工程显著提高了产量水平。在呼吸活性监测系统中，最佳菌株产生了3.54mg/L的维生素B。通过单因素和田口方法对发酵培养基进行进一步优化，产量显著增加，在含有低成本限定培养基的5L发酵罐中达到了21.09mg/L的显著产量。这些发现证明了工程化……菌株用于工业生产维生素B的潜力，提供了一种经济高效的生物技术解决方案。
