Enhancing Vitamin B Production in Engineered through Cofactor Engineering and Fermentation Media Optimization.

Vitamin B has garnered increasing interest in biotechnological applications due to its critical role in human health and economic benefits. In this study, heterologous vitamin B biosynthetic pathway genes were integrated into the chromosome of . Cofactor engineering significantly enhanced production levels by expressing the hemoglobin gene from for improved oxygen transfer and substituting the Embden-Meyerhof-Parnas pathway with the Entner-Doudoroff pathway to increase ATP and NADPH availability. The optimal strain produced 3.54 mg/L of vitamin B in a respiration activity monitoring system. Further optimization of fermentation media through single-factor and Taguchi methods resulted in a significant increase in production, achieving a remarkable 21.09 mg/L in a 5 L fermenter containing a low-cost defined medium. These findings demonstrate the potential of engineered strains for industrial vitamin B production, offering a cost-effective and efficient biotechnological solution.