Kong Lingming, Zhang Liang
Treatment & Prevention Center for Mental Disorder, No. 904th Hospital, Changzhou, Jiangsu, 213003, People's Republic of China.
Psychiatry Department, The 5th People's Hospital of Luoyang, Luoyang, Henan, 471027, People's Republic of China.
Neuropsychiatr Dis Treat. 2025 Apr 4;21:729-739. doi: 10.2147/NDT.S511375. eCollection 2025.
Symptomatic diagnosis combined with unclear pathological mechanism and diverse etiology may increase misdiagnosis risk for generalized anxiety disorder (GAD) in the clinical setting. This study aimed to confirm the diagnostic value of aberrantly expressed long non-coding RNAs (lncRNAs) for GAD.
Eighty sex- and age-matched patients with GAD and major depressive disorder (MDD) and healthy controls (HCs) were enrolled using a convenient sampling method. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify lncRNA expression levels in all participants, and a receiver operating characteristic (ROC) curve was used to test the accuracy of aberrantly expressed lncRNAs in differentiating health conditions.
ΔCt values of ENST00000505825, NONHSAG017299, NONHSAT078768, NONHSAT029028, NONHSAT101077, NONHSAT031726, TCONS_l2_00010607 in GAD patients were less than in HCs (<0.05 or 0.01). The total severity score of HAMA-14 and somatic anxiety scores were negatively correlated with ΔCt values of ENST00000505825, NONHSAG017299, NONHSAT078768, NONHSAT029028, NONHSAT101077, NONHSAT031726, ENST00000505825, TCONS_l2_00010607, and NONHSAT131696, and psychic anxiety scores were negatively associated with the ΔCt value of ENST00000505825 (<0.05 or 0.01). The AUC of the combined ROC curve between patients with GAD and healthy people was 0.810, with sensitivity and specificity of 0.825 and 0.762, respectively (<0.05 or 0.01). The AUC of the combined ROC curve between patients with GAD and MDD patients was 0.938, with sensitivity and specificity of 0.850 and 0.900, respectively (<0.05 or 0.01).
ENST00000505825, NONHSAG017299, NONHSAT078768, NONHSAT029028, NONHSAT101077, NONHSAT031726, TCONS_l2_00010607, which may serve as biomarkers for the GAD diagnosis and differentiation between GAD and MDD, can improve the diagnostic accuracy and avoid misdiagnosis to a certain extent. It is also beneficial for personalized treatment of GAD.
在临床环境中，症状诊断结合不明确的病理机制和多样的病因可能会增加广泛性焦虑症（GAD）的误诊风险。本研究旨在确认异常表达的长链非编码RNA（lncRNA）对GAD的诊断价值。
采用便利抽样法纳入80例年龄和性别匹配的GAD患者、重度抑郁症（MDD）患者和健康对照者（HCs）。运用定量实时聚合酶链反应（qRT-PCR）验证所有参与者的lncRNA表达水平，并使用受试者工作特征（ROC）曲线检验异常表达的lncRNA在区分健康状况方面的准确性。
GAD患者中ENST00000505825、NONHSAG017299、NONHSAT078768、NONHSAT029028、NONHSAT101077、NONHSAT031726、TCONS_l2_00010607的ΔCt值低于HCs（<0.05或0.01）。汉密尔顿焦虑量表（HAMA-14）的总严重程度评分和躯体焦虑评分与ENST00000505825、NONHSAG017299、NONHSAT078768、NONHSAT029028、NONHSAT101077、NONHSAT031726、ENST00000505825、TCONS_l2_00010607以及NONHSAT131696的ΔCt值呈负相关，精神焦虑评分与ENST00000505825的ΔCt值呈负相关（<0.05或0.01）。GAD患者与健康人联合ROC曲线的AUC为0.810，敏感性和特异性分别为0.825和0.762（<0.05或0.01）。GAD患者与MDD患者联合ROC曲线的AUC为0.938，敏感性和特异性分别为0.850和0.900（<0.05或0.01）。
ENST00000505825、NONHSAG017299、NONHSAT078768、NONHSAT029028、NONHSAT101077、NONHSAT031726、TCONS_l2_00010607可作为GAD诊断及GAD与MDD鉴别的生物标志物，能在一定程度上提高诊断准确性并避免误诊，也有利于GAD的个体化治疗。
