Chen Sheng-Dong, Sun Xin-Yang, Niu Wei, Kong Ling-Ming, He Ming-Jun, Fan Hui-Min, Li Wan-Shuai, Zhong Ai-Fang, Zhang Li-Yi, Lu Jim
Department of Psychiatry and Psychology, Second Military Medical University, Shanghai 200433, People's Republic of China; Department of Neurology, No.102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu 213003, People's Republic of China.
Department of Psychiatry and Psychology, PingAn Health Cloud Company Ltd. of China, Shanghai 200030, People's Republic of China.
Compr Psychiatry. 2016 Aug;69:216-24. doi: 10.1016/j.comppsych.2016.05.006. Epub 2016 May 10.
This study investigated the correlation between the level of microRNA expression in peripheral blood mononuclear cells (PBMCs) and symptomatology in patients with generalized anxiety disorder (GAD). MicroRNA array was performed in peripheral blood mononuclear cells (PBMCs) obtained from GAD patients with gender, age, ethnicity-matched healthy controls. Then real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to verify the top 7 miRNAs with the highest fold-change values in 76 GAD patients and 39 healthy controls. It demonstrated that 5 miRNAs showed significantly differences in expression levels (P<0.01). These 5 GAD-associated miRNAs were finally selected into our study to analyze the association between the plasma level of miRNAs expression and symptomatology scores in Hamilton Anxiety Scale (HAMA). Results showed that the level of miR-4505 and miR-663 was negatively correlated with the total HAMA scores in GAD patients (r=0.2228, r=0.264 P<0.05). MiR-663 was selected into the regression equation of HAMA total scores and psychic anxiety symptomatology scores, and it could explain 5.3% of the HAMA total scores and 15.3% of the anxiety symptomatology scores. This study analyzed preliminarily possible circulating miRNAs expression changes in GAD patients, and the expression level of miR-663 highly correlated with psychic anxiety symptoms, further molecular mechanism of which needs to be explored.
本研究调查了广泛性焦虑障碍（GAD）患者外周血单个核细胞（PBMCs）中微小RNA表达水平与症状学之间的相关性。对从性别、年龄、种族匹配的健康对照的GAD患者获取的外周血单个核细胞进行微小RNA芯片检测。然后，使用实时定量逆转录聚合酶链反应（qRT-PCR）对76例GAD患者和39例健康对照中折叠变化值最高的前7种微小RNA进行验证。结果显示，5种微小RNA的表达水平存在显著差异（P<0.01）。最终选择这5种与GAD相关的微小RNA纳入我们的研究，以分析微小RNA表达的血浆水平与汉密尔顿焦虑量表（HAMA）症状学评分之间的关联。结果显示，miR-4505和miR-663的水平与GAD患者的HAMA总分呈负相关（r=0.2228，r=0.264，P<0.05）。MiR-663被纳入HAMA总分和精神焦虑症状学评分的回归方程，它可解释HAMA总分的5.3%和焦虑症状学评分的15.3%。本研究初步分析了GAD患者可能的循环微小RNA表达变化，且miR-663的表达水平与精神焦虑症状高度相关，其进一步的分子机制有待探索。
