Mabrouk Shreifa G, El-Nokrashy Asmaa M, Ebied Nahla A, Abdella Bahaa H, Zayed Mohamed M, Aboleila Sayed M, Mohamed Radi A
Department of Aquaculture, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafr El-Sheikh, Egypt.
Food Hygiene Unit, Animal Health Research Institute, Provisional Lab Kafr El-Sheikh, Agricultural Research Center (ARC), Kafr El-Sheikh, Egypt.
Open Vet J. 2025 Feb;15(2):746-764. doi: 10.5455/OVJ.2025.v15.i2.24. Epub 2025 Feb 28.
Numerous natural phytobiotic products are used as feed additives to enhance fish performance, quality, and immunity.
This study evaluated the effect of a natural phytobiotics mixture [Syrena Boost (SB)] on growth performance, intestine health, immune-oxidative status, and hemato-biochemical parameters of fingerlings ().
Fish ( = 4,800, average initial weight = 1.247 ± 0.047 g) were randomly distributed in 12 aquatic compartments with a total water volume of 2 m3 (2 × 1 × 1 m) for each one, representing four groups, in triplicate: control group (CG), SB1, SB2, and SB3, in which fish received a basal diet (30/6, protein/lipid ratio) containing 0, 0.1, 0.2, 0.4 g kg-1 SB, respectively, for 60 days.
The results showed a significant ( < 0.05) improvement in fish performance (survival rate, specific growth rate, weight gain, final weight, and feed conversion ratio) and intestine histomorphology (increased goblet cell number, intestinal villi length, but inter-villi space, decreased) in fish that received SB1, SB2, and SB3 compared with CG ( < 0.05) with the most favorable outcomes observed in treatment supplemented with SB2. Dietary inclusion of SB at different doses (SB1, SB2, and SB3) improved certain blood hematological parameters, including globulin and total protein, while decreasing aspartate aminotransferase, alanine aminotransferase, cholesterol, triglycerides, glucose, and albumin compared with CG ( < 0.05), with the most favorable outcomes observed in SB2. The fish showed improvements in digestive enzymes (lipase and amylase), antioxidant enzymes (increased catalase, superoxide dismutase), as well as a reduction in malondialdehyde, as well as showed improvement in the immunity health indicators (phagocytic index, phagocytic activity, and lysozyme activity), and gene expression (glutathione peroxidase, and catalase, tumor necrosis factor-alpha, interleukin-1β, insulin-like growth factor, interleukin-8, and growth hormone receptor) when they received SB1, SB2, and SB3 supplemented diets, with the most favorable outcomes observed in SB2 compared with CG ( < 0.05). Thus, the SB at 0.2 g kg-1 diet (SB2) can be used effectively in Tilapia diets to improve growth, intestinal health, blood health, oxidative status, and immune-related gene expression.
Dietary supplementation with a natural phytobiotic mixture (SB®) improved fish performance, intestine health, and the immune-physiological status of Nile tilapia fingerlings. The best findings in this experiment were observed in fish that received SB2.
众多天然植物源生物产品被用作饲料添加剂，以提高鱼类的生长性能、品质和免疫力。
本研究评估了一种天然植物源生物混合物[Syrena Boost（SB）]对鱼苗生长性能、肠道健康、免疫氧化状态和血液生化参数的影响。
将4800尾鱼（平均初始体重=1.247±0.047克）随机分配到12个水族箱中，每个水族箱总体积为2立方米（2×1×1米），分为四组，每组三个重复：对照组（CG）、SB1、SB2和SB3，其中鱼分别接受含0、0.1、0.2、0.4克/千克SB的基础饲料（蛋白质/脂肪比为30/6），持续60天。
结果显示，与CG组相比，接受SB1、SB2和SB3的鱼在生长性能（存活率、特定生长率、体重增加、终末体重和饲料转化率）和肠道组织形态学方面表现出显著（P<0.05）改善（杯状细胞数量增加、肠绒毛长度增加，但绒毛间隙减小）（P<0.05），在添加SB2的处理组中观察到最有利的结果。与CG组相比，不同剂量（SB1、SB2和SB3）的饲料中添加SB可改善某些血液学参数，包括球蛋白和总蛋白，同时降低天冬氨酸转氨酶、丙氨酸转氨酶、胆固醇、甘油三酯、葡萄糖和白蛋白（P<0.05），在SB2组中观察到最有利的结果。接受添加SB1、SB2和SB3饲料的鱼在消化酶（脂肪酶和淀粉酶）、抗氧化酶（过氧化氢酶、超氧化物歧化酶增加）方面有所改善，丙二醛减少，免疫健康指标（吞噬指数、吞噬活性和溶菌酶活性）以及基因表达（谷胱甘肽过氧化物酶、过氧化氢酶、肿瘤坏死因子-α、白细胞介素-1β、胰岛素样生长因子、白细胞介素-8和生长激素受体）也有所改善，与CG组相比，SB2组观察到最有利的结果（P<0.05）。因此，饲料中添加0.2克/千克的SB（SB2）可有效用于罗非鱼饲料中，以改善生长、肠道健康、血液健康、氧化状态和免疫相关基因表达。
饲料中添加天然植物源生物混合物（SB®）可改善尼罗罗非鱼苗的生长性能、肠道健康和免疫生理状态。本实验中，在接受SB2的鱼中观察到最佳结果。
