Abdul-Latif Saif Aldeen Kamal, Yousif Afaf Abdulrahman
Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
Open Vet J. 2025 Feb;15(2):731-737. doi: 10.5455/OVJ.2025.v15.i2.22. Epub 2025 Feb 28.
Strangles is a highly contagious equine respiratory disease caused by . It is a globally significant pathogen and one of the most common infectious agents in horses. In Iraq, no sequencing data on this pathogen are available, and only two molecular studies have been published to date. This study provides preliminary insights into strain diversity and provides a foundation for future large-scale investigations.
This study aimed to investigate the molecular characteristics, identify gene alleles, and perform a phylogenetic analysis of isolates from horses in Baghdad, Iraq.
We analyzed 59 . isolates previously obtained from horses clinical samples. Conventional PCR (Polymerase Chain Reaction) targeting the gene was used to identify . Additionally, nine PCR- positive gene products were sequenced, followed by phylogenetic analysis and allele identification.
We confirmed 49 isolates as from the 59 isolates according to the molecular assay. Additionally, nine PCR products were used for sequencing and allele typing of the gene, which provided the initial report of -97 allele identification in Iraq. Phylogenetic analysis along with gene typing revealed a close relationship between the Iraqi strains and one Iranian strain with 100% sequence identity, revealing important epidemiological relationships that may indicate regional ties to the strain detected in Iran.
The present study represents the first investigation of allele typing in Iraq, identifying the -97 allele of along with its unique amino acid variations. The findings highlight genetic similarities between Iraqi isolates and a strain from Iran, suggesting the potential regional dissemination of .
马腺疫是一种由[病原体名称未给出]引起的高度传染性马呼吸道疾病。它是一种具有全球重要性的病原体，也是马中最常见的传染源之一。在伊拉克，尚无关于该病原体的测序数据，迄今为止仅发表了两项分子研究。本研究为菌株多样性提供了初步见解，并为未来的大规模调查奠定了基础。
本研究旨在调查来自伊拉克巴格达马匹的[病原体名称未给出]分离株的分子特征，鉴定[基因名称未给出]基因等位基因，并进行系统发育分析。
我们分析了先前从马临床样本中获得的59株[病原体名称未给出]分离株。使用针对[基因名称未给出]基因的常规聚合酶链反应（PCR）来鉴定[病原体名称未给出]。此外，对9个PCR阳性的[基因名称未给出]基因产物进行了测序，随后进行了系统发育分析和等位基因鉴定。
根据分子检测，我们从59株分离株中确认了49株为[病原体名称未给出]。此外，9个PCR产物用于[基因名称未给出]基因的测序和等位基因分型，这提供了伊拉克-97等位基因鉴定的初步报告。系统发育分析以及[基因名称未给出]基因分型显示伊拉克菌株与一株伊朗菌株之间存在密切关系，序列同一性为100%，揭示了重要的流行病学关系，这可能表明与在伊朗检测到的菌株存在区域联系。
本研究是伊拉克首次对[病原体名称未给出]等位基因分型进行的调查，鉴定出了[病原体名称未给出]的-97等位基因及其独特的氨基酸变异。研究结果突出了伊拉克分离株与一株伊朗菌株之间的遗传相似性，表明[病原体名称未给出]可能在区域内传播。
