Wang Jiaming, Liu Mingchong, Yang Chensong, Huang Xixi, Lin Chenyu, Zhu Yuqian, Wang Jing, Zhang Hongyi, Chen Bingdi, Sun Guixin
Department of Traumatic Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, China.
The Institute for Translational Nanomedicine, Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, School of Medicine, Tongji University, Shanghai, China.
J Biomater Sci Polym Ed. 2025 Apr 9:1-23. doi: 10.1080/09205063.2025.2489846.
Black phosphorus nanosheets (BP) have been widely used in bone tissue engineering due to their superior properties and good biocompatibility, as well as the fact that they are composed of only a single element of phosphorus, which is highly homologous to the inorganic components of natural bone. Hydrogels based on gelatin methacryloyl (GelMA)/hyaluronic acid methacryloyl (HAMA) composites were prepared by photocrosslinking. The physicochemical properties of the prepared GelMA/HAMA, GelMA/HAMA/BP, GelMA/HAMA/BMP and GelMA/HAMA/BP@BMP hydrogels were characterized using Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). In addition, the mechanical properties, swelling rate and degradation rate of the hydrogels were investigated, and the results showed that GelMA/HAMA/BP@BMP possessed good physicochemical and mechanical properties, which were suitable for cell adhesion growth and proliferation. BP as a carrier could ensure the stable and sustained release of the bioactive factor: bone morphogenetic protein 2 (BMP), which could promote the recruitment and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and its degradation and binding to surrounding calcium ions can promote biomineralization. Therefore, this BMP-modified black phosphorus hydrogel system provides a new strategy for bone regeneration.
黑磷纳米片（BP）因其优异的性能、良好的生物相容性，以及仅由单一磷元素组成且与天然骨的无机成分高度同源，已被广泛应用于骨组织工程。通过光交联制备了基于甲基丙烯酰化明胶（GelMA）/甲基丙烯酰化透明质酸（HAMA）复合材料的水凝胶。使用傅里叶变换红外光谱（FTIR）、动态光散射（DLS）、透射电子显微镜（TEM）和扫描电子显微镜（SEM）对制备的GelMA/HAMA、GelMA/HAMA/BP、GelMA/HAMA/BMP和GelMA/HAMA/BP@BMP水凝胶的物理化学性质进行了表征。此外，研究了水凝胶的力学性能、溶胀率和降解率，结果表明GelMA/HAMA/BP@BMP具有良好的物理化学和力学性能，适合细胞黏附生长和增殖。BP作为载体可确保生物活性因子骨形态发生蛋白2（BMP）的稳定和持续释放，其可促进骨髓间充质干细胞（BMSCs）的募集和成骨分化，并且其降解以及与周围钙离子的结合可促进生物矿化。因此，这种BMP修饰的黑磷水凝胶系统为骨再生提供了一种新策略。
