Zhang Yuzhu, Wang Yu, Gu Yuan, Liu Yang, Liu Guohua, Wu Jun, Bai Nan
Intensive care unit, LinYi People's Hospital, LinYi, Shandong, China.
Medical Cosmetology and Plastic Surgery Center, LinYi People's Hospital, LinYi, Shandong, China.
Sci Rep. 2025 Apr 9;15(1):12167. doi: 10.1038/s41598-025-85439-8.
To explore the regulatory effect of c-Jun N-terminal kinase (JNK) inhibitor (SP600125) on forkhead box protein L2 (FOXL2) gene in human ovarian granulosa cell tumor cells (KGN cells). The main pathogenic gene FOXL2 of ovarian cancer was screened by bioinformatics method. KGN cells were randomly divided into control group and experimental group. Different concentrations of SP600125 (0.1, 1, 5, 10, 50 µM) were added to the experimental group, and an equal volume of dimethyl sulfoxide (DMSO) was added to the control group. The cells were incubated for 48 h. Cell RNA was extracted and reverse transcribed into cDNA. The mRNA expression level of FOXL2 was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Proteins were extracted, and the expression level of FOXL2 protein was detected by Western blot. The proliferation ability of KGN cells treated with SP600125 was detected by MTT assay. Cell scratch assay was used to detect its migration ability. Different concentrations of JNK inhibitor reduced the expression of FOXL2 in ovarian granulosa cells KGN, and 1 µM had the best inhibitory effect. JNK inhibitor reduces the expression of FOXL2 in ovarian granulosa cell tumor KGN.
探讨c-Jun氨基末端激酶（JNK）抑制剂（SP600125）对人卵巢颗粒细胞瘤细胞（KGN细胞）中叉头框蛋白L2（FOXL2）基因的调控作用。采用生物信息学方法筛选卵巢癌主要致病基因FOXL2。将KGN细胞随机分为对照组和实验组。实验组加入不同浓度的SP600125（0.1、1、5、10、50μM），对照组加入等体积的二甲基亚砜（DMSO）。细胞孵育48小时。提取细胞RNA并逆转录为cDNA。通过定量逆转录聚合酶链反应（qRT-PCR）检测FOXL2的mRNA表达水平。提取蛋白质，通过蛋白质印迹法检测FOXL2蛋白的表达水平。采用MTT法检测经SP600125处理的KGN细胞的增殖能力。采用细胞划痕试验检测其迁移能力。不同浓度的JNK抑制剂降低了卵巢颗粒细胞KGN中FOXL2的表达，1μM的抑制效果最佳。JNK抑制剂降低了卵巢颗粒细胞瘤KGN中FOXL2的表达。
